气虚痰湿型肺腺癌生物标志物的鉴定

IF 1.9 4区 医学 Q3 RESPIRATORY SYSTEM Clinical Respiratory Journal Pub Date : 2024-08-06 DOI:10.1111/crj.13812
Jiabin Chen, Sheng Wang, Qiaolei Yang, Yongjun Zhang, Jianfei Shen, Kequn Chai
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引用次数: 0

摘要

背景:气虚痰湿证(QPD)是肺腺癌(LUAD)最常见的中医证候之一。本研究旨在确定肺腺癌气虚痰湿证的特异性生物标志物:方法:收集患有 QPD 的 LUAD 患者、患有非 QPD 的 LUAD 患者(N-QPD)和健康对照(H)的外周血单核细胞(PBMCs),并进行 RNA-seq 分析,以确定差异表达基因(DEGs)。计算每个 DEG 的接收者操作特征曲线下面积(AUC),并通过 qRT-PCR 验证 AUC 最高的前 10 个 DEG。利用逻辑回归分析建立了一个用AUC评估的诊断模型:结果:共有 135 人参加了这项研究(训练集:15 个 QPD、15 个 N-QPD、15 个 N-QPD):结果:共有 135 人参加了这项研究(训练集:15 QPD、15 N-QPD、15 H;验证集:30 QPD、30 N-QPD、30 H):30个QPD、30个N-QPD、30个H)。在 QPD 和 N-QPD 之间共鉴定出 1480 个 DEGs。qRT-PCR 结果显示,DDR2 表达下调,PPARG 表达上调,这与训练集的结果一致。我们利用这两个基因建立了一个诊断模型。在训练组和验证组中,诊断模型的AUC分别为0.891和0.777:结论:我们发现了两个基因(DDR2和PPARG)是LUAD伴QPD综合征的综合征特异性生物标志物,并建立了一个新的诊断模型,这可能有助于提高临床诊断的准确性和敏感性,并为LUAD的天然药物治疗提供新的靶点。
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Identification of Biomarkers for Lung Adenocarcinoma With Qi Deficiency and Phlegm Dampness

Background

Qi deficiency and phlegm dampness (QPD) is one of the most common traditional Chinese medicine (TCM) syndromes in lung adenocarcinoma (LUAD). This study aimed to identify syndrome-specific biomarkers for LUAD with QPD syndrome.

Methods

Peripheral blood mononuclear cells (PBMCs) from LUAD patients with QPD, LUAD patients with non-QPD (N-QPD), and healthy control (H) were collected and analyzed with RNA-seq to identify differentially expressed genes (DEGs). The area under the receiver operator characteristic curve (AUC) of each DEG was calculated, and the top 10 highest AUC DEGs were validated by qRT-PCR. Logistic regression analysis was used to develop a diagnostic model evaluated with AUC.

Results

A total of 135 individuals were enrolled in this study (training set: 15 QPD, 15 N-QPD, 15 H; validation set: 30 QPD, 30 N-QPD, 30 H). A total of 1480 DEGs were identified between QPD and N-QPD. The qRT-PCR results showed that the expression of DDR2 was downregulated, and PPARG was upregulated, which was in line with the finding of the training set. We developed a diagnostic model with these two genes. The AUC of the diagnostic model in the training cohort and validation cohort was 0.891 and 0.777, respectively.

Conclusions

We identified the two genes (DDR2 and PPARG) as syndrome-specific biomarkers for LUAD with QPD syndrome and developed a novel diagnostic model, which may help to improve the accuracy and sensibility of clinical diagnosis and provide a new target for natural drug treatment of LUAD.

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来源期刊
Clinical Respiratory Journal
Clinical Respiratory Journal 医学-呼吸系统
CiteScore
3.70
自引率
0.00%
发文量
104
审稿时长
>12 weeks
期刊介绍: Overview Effective with the 2016 volume, this journal will be published in an online-only format. Aims and Scope The Clinical Respiratory Journal (CRJ) provides a forum for clinical research in all areas of respiratory medicine from clinical lung disease to basic research relevant to the clinic. We publish original research, review articles, case studies, editorials and book reviews in all areas of clinical lung disease including: Asthma Allergy COPD Non-invasive ventilation Sleep related breathing disorders Interstitial lung diseases Lung cancer Clinical genetics Rhinitis Airway and lung infection Epidemiology Pediatrics CRJ provides a fast-track service for selected Phase II and Phase III trial studies. Keywords Clinical Respiratory Journal, respiratory, pulmonary, medicine, clinical, lung disease, Abstracting and Indexing Information Academic Search (EBSCO Publishing) Academic Search Alumni Edition (EBSCO Publishing) Embase (Elsevier) Health & Medical Collection (ProQuest) Health Research Premium Collection (ProQuest) HEED: Health Economic Evaluations Database (Wiley-Blackwell) Hospital Premium Collection (ProQuest) Journal Citation Reports/Science Edition (Clarivate Analytics) MEDLINE/PubMed (NLM) ProQuest Central (ProQuest) Science Citation Index Expanded (Clarivate Analytics) SCOPUS (Elsevier)
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