探索确定的人类肠道微生物群联合体的恢复力和稳定性:等温微量热法研究

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2024-08-08 DOI:10.1002/mbo3.1430
Anna Kattel, Valter Aro, Petri-Jaan Lahtvee, Jekaterina Kazantseva, Arvi Jõers, Ranno Nahku, Isma Belouah
{"title":"探索确定的人类肠道微生物群联合体的恢复力和稳定性:等温微量热法研究","authors":"Anna Kattel,&nbsp;Valter Aro,&nbsp;Petri-Jaan Lahtvee,&nbsp;Jekaterina Kazantseva,&nbsp;Arvi Jõers,&nbsp;Ranno Nahku,&nbsp;Isma Belouah","doi":"10.1002/mbo3.1430","DOIUrl":null,"url":null,"abstract":"<p>The gut microbiota significantly contributes to human health and well-being. The aim of this study was to evaluate the stability and resilience of a consortium composed of three next-generation probiotics (NGPs) candidates originally found in the human gut. The growth patterns of <i>Akkermansia muciniphila</i>, <i>Bacteroides thetaiotaomicron</i>, and <i>Faecalibacterium prausnitzii</i> were studied both individually and consortium. The growth kinetics of Akkermansia muciniphila (<i>A. muciniphila</i>), Bacteroides thetaiotaomicron (<i>B. thetaiotaomicron</i>), and Faecalibacterium prausnitzii (<i>F. prausnitzii</i>) were characterized both individually and in consortium using isothermal microcalorimetry and 16S ribosomal RNA next-generation sequencing. The consortium reached stability after three passages and demonstrated resilience to changes in its initial composition. The concentration of butyrate produced was nearly twice as high in the consortium compared to the monoculture of F. prausnitzii. The experimental conditions and methodologies used in this article are a solid foundation for developing further complex consortia.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11307317/pdf/","citationCount":"0","resultStr":"{\"title\":\"Exploring the resilience and stability of a defined human gut microbiota consortium: An isothermal microcalorimetric study\",\"authors\":\"Anna Kattel,&nbsp;Valter Aro,&nbsp;Petri-Jaan Lahtvee,&nbsp;Jekaterina Kazantseva,&nbsp;Arvi Jõers,&nbsp;Ranno Nahku,&nbsp;Isma Belouah\",\"doi\":\"10.1002/mbo3.1430\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The gut microbiota significantly contributes to human health and well-being. The aim of this study was to evaluate the stability and resilience of a consortium composed of three next-generation probiotics (NGPs) candidates originally found in the human gut. The growth patterns of <i>Akkermansia muciniphila</i>, <i>Bacteroides thetaiotaomicron</i>, and <i>Faecalibacterium prausnitzii</i> were studied both individually and consortium. The growth kinetics of Akkermansia muciniphila (<i>A. muciniphila</i>), Bacteroides thetaiotaomicron (<i>B. thetaiotaomicron</i>), and Faecalibacterium prausnitzii (<i>F. prausnitzii</i>) were characterized both individually and in consortium using isothermal microcalorimetry and 16S ribosomal RNA next-generation sequencing. The consortium reached stability after three passages and demonstrated resilience to changes in its initial composition. The concentration of butyrate produced was nearly twice as high in the consortium compared to the monoculture of F. prausnitzii. The experimental conditions and methodologies used in this article are a solid foundation for developing further complex consortia.</p>\",\"PeriodicalId\":18573,\"journal\":{\"name\":\"MicrobiologyOpen\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-08-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11307317/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"MicrobiologyOpen\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/mbo3.1430\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"MicrobiologyOpen","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mbo3.1430","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

肠道微生物群对人类的健康和福祉有重大贡献。本研究旨在评估由最初在人类肠道中发现的三种候选下一代益生菌(NGPs)组成的联合体的稳定性和恢复力。研究了 Akkermansia muciniphila、Bacteroides thetaiotaomicron 和 Faecalibacterium prausnitzii 的单独和联合生长模式。利用等温微量热仪和 16S 核糖体 RNA 下一代测序技术,研究了 Akkermansia muciniphila(A. muciniphila)、Bacteroides thetaiotaomicron(B. thetaiotaomicron)和 Faecalibacterium prausnitzii(F. prausnitzii)单个和联合体的生长动力学特征。联合体在经过三次培养后达到稳定,并显示出对初始成分变化的适应能力。与单培养的 F. prausnitzii 相比,联合体产生的丁酸盐浓度高出近两倍。本文中使用的实验条件和方法为进一步开发复杂的联合菌群奠定了坚实的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Exploring the resilience and stability of a defined human gut microbiota consortium: An isothermal microcalorimetric study

The gut microbiota significantly contributes to human health and well-being. The aim of this study was to evaluate the stability and resilience of a consortium composed of three next-generation probiotics (NGPs) candidates originally found in the human gut. The growth patterns of Akkermansia muciniphila, Bacteroides thetaiotaomicron, and Faecalibacterium prausnitzii were studied both individually and consortium. The growth kinetics of Akkermansia muciniphila (A. muciniphila), Bacteroides thetaiotaomicron (B. thetaiotaomicron), and Faecalibacterium prausnitzii (F. prausnitzii) were characterized both individually and in consortium using isothermal microcalorimetry and 16S ribosomal RNA next-generation sequencing. The consortium reached stability after three passages and demonstrated resilience to changes in its initial composition. The concentration of butyrate produced was nearly twice as high in the consortium compared to the monoculture of F. prausnitzii. The experimental conditions and methodologies used in this article are a solid foundation for developing further complex consortia.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
期刊最新文献
Listeria monocytogenes in the seafood industry: Exploring contamination sources, outbreaks, antibiotic susceptibility and genetic diversity Influence of microbiota on the growth and gene expression of Clostridioides difficile in an in vitro coculture model The metagenomic landscape of a high-altitude geothermal spring in Tajikistan reveals a novel Desulfurococcaceae member, Zestomicrobium tamdykulense gen. nov., sp. nov High-quality genome of a novel Thermosynechococcaceae species from Namibia and characterization of its protein expression patterns at elevated temperatures The effects of stress on gut virome: Implications on infectious disease and systemic disorders
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1