模拟骨质疏松症骨转移的骨芯片。

IF 8.2 2区 医学 Q1 ENGINEERING, BIOMEDICAL Biofabrication Pub Date : 2024-08-14 DOI:10.1088/1758-5090/ad6cf9
Sunghan Lee, Young Gyun Kim, Hyo-Il Jung, Ji Seok Lim, Ki Chang Nam, Han Seok Choi, Bong Seop Kwak
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引用次数: 0

摘要

骨质疏松症是最常见的骨骼疾病,是一种高度危险的疾病,可促进骨转移。由于目前治疗骨质疏松症的方法包括长期药物治疗,而治疗骨转移的方法尚不清楚,因此需要不断努力开发治疗骨质疏松症的药物。传统上用于药物开发的动物实验会引发伦理问题,而且昂贵、耗时。器官芯片技术正被开发为补充此类动物模型的工具。在这项研究中,我们在细胞外基质(ECM)环境中共同培养成骨细胞、骨细胞和破骨细胞,开发出了一种芯片上的骨。然后,我们利用乳腺癌细胞模拟了三种不同骨质条件下的骨转移,观察到骨质疏松条件下的骨转移最为活跃。此外,研究还发现,骨质疏松条件下骨转移的促进是由于血管通透性增加。本研究开发的芯片骨可以作为骨质疏松症和骨转移药物开发动物模型的补充平台。
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Bone-on-a-chip simulating bone metastasis in osteoporosis.

Osteoporosis is the most common bone disorder, which is a highly dangerous condition that can promote bone metastases. As the current treatment for osteoporosis involves long-term medication therapy and a cure for bone metastasis is not known, ongoing efforts are required for drug development for osteoporosis. Animal experiments, traditionally used for drug development, raise ethical concerns and are expensive and time-consuming. Organ-on-a-chip technology is being developed as a tool to supplement such animal models. In this study, we developed a bone-on-a-chip by co-culturing osteoblasts, osteocytes, and osteoclasts in an extracellular matrix environment that can represent normal bone, osteopenia, and osteoporotic conditions. We then simulated bone metastases using breast cancer cells in three different bone conditions and observed that bone metastases were most active in osteoporotic conditions. Furthermore, it was revealed that the promotion of bone metastasis in osteoporotic conditions is due to increased vascular permeability. The bone-on-a-chip developed in this study can serve as a platform to complement animal models for drug development for osteoporosis and bone metastasis.

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来源期刊
Biofabrication
Biofabrication ENGINEERING, BIOMEDICAL-MATERIALS SCIENCE, BIOMATERIALS
CiteScore
17.40
自引率
3.30%
发文量
118
审稿时长
2 months
期刊介绍: Biofabrication is dedicated to advancing cutting-edge research on the utilization of cells, proteins, biological materials, and biomaterials as fundamental components for the construction of biological systems and/or therapeutic products. Additionally, it proudly serves as the official journal of the International Society for Biofabrication (ISBF).
期刊最新文献
Bioinks for engineering gradient-based osteochondral and meniscal tissue substitutes: a review. Current progress ofin vitrovascular models on microfluidic chips. Enhanced osteogenic differentiation in hyaluronic acid methacrylate (HAMA) matrix: a comparative study of hPDC and hBMSC spheroids for bone tissue engineering. Advancement of 3D biofabrication in repairing and regeneration of cartilage defects. One-step bioprinting of endothelialized, self-supporting arterial and venous networks.
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