Adin Sejdic , Hans Jakob Hartling , Jon Gitz Holler , Lars Klingen Gjærde , Arnold Matovu Dungu , Maria Elizabeth Engel Møller , Rebecca Svanberg Teglgaard , Carsten Utoft Utoft Niemann , Patrick Terrence Brooks , Trine H. Mogensen , Nina Weis , Daria Podlekareva , Marie Louise Baum Jørgensen , Anne Ortved Gang , Ditte Stampe Hersby , Annemette Hald , Susanne Dam Nielsen , Anne-Mette Lebech , Marie Helleberg , Jens Lundgren , Birgitte Lindegaard
{"title":"COVID-19住院患者入院时按体重指数分层的深度免疫细胞表型和诱导免疫细胞反应：一项观察性多中心队列试点研究。","authors":"Adin Sejdic , Hans Jakob Hartling , Jon Gitz Holler , Lars Klingen Gjærde , Arnold Matovu Dungu , Maria Elizabeth Engel Møller , Rebecca Svanberg Teglgaard , Carsten Utoft Utoft Niemann , Patrick Terrence Brooks , Trine H. Mogensen , Nina Weis , Daria Podlekareva , Marie Louise Baum Jørgensen , Anne Ortved Gang , Ditte Stampe Hersby , Annemette Hald , Susanne Dam Nielsen , Anne-Mette Lebech , Marie Helleberg , Jens Lundgren , Birgitte Lindegaard","doi":"10.1016/j.clim.2024.110336","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.</p></div><div><h3>Methods and results</h3><p>This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.</p></div><div><h3>Conclusions</h3><p>Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.</p></div>","PeriodicalId":10392,"journal":{"name":"Clinical immunology","volume":"267 ","pages":"Article 110336"},"PeriodicalIF":4.5000,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1521661624004455/pdfft?md5=ef1ffd1c122f7cb2e1e63c9acfb958cd&pid=1-s2.0-S1521661624004455-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study\",\"authors\":\"Adin Sejdic , Hans Jakob Hartling , Jon Gitz Holler , Lars Klingen Gjærde , Arnold Matovu Dungu , Maria Elizabeth Engel Møller , Rebecca Svanberg Teglgaard , Carsten Utoft Utoft Niemann , Patrick Terrence Brooks , Trine H. Mogensen , Nina Weis , Daria Podlekareva , Marie Louise Baum Jørgensen , Anne Ortved Gang , Ditte Stampe Hersby , Annemette Hald , Susanne Dam Nielsen , Anne-Mette Lebech , Marie Helleberg , Jens Lundgren , Birgitte Lindegaard\",\"doi\":\"10.1016/j.clim.2024.110336\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.</p></div><div><h3>Methods and results</h3><p>This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.</p></div><div><h3>Conclusions</h3><p>Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.</p></div>\",\"PeriodicalId\":10392,\"journal\":{\"name\":\"Clinical immunology\",\"volume\":\"267 \",\"pages\":\"Article 110336\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-08-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1521661624004455/pdfft?md5=ef1ffd1c122f7cb2e1e63c9acfb958cd&pid=1-s2.0-S1521661624004455-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1521661624004455\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical immunology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1521661624004455","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study
Introduction
Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.
Methods and results
This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.
Conclusions
Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.
期刊介绍：
Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.
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