Somu Yadav, Stuti Bhagat, Sanjay Singh, Pawan Kumar Maurya
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引用次数: 0
摘要
红细胞在人体衰老和老年相关疾病的过程中会发生一些变化,因此被作为衰老过程的生物标志物进行研究。本研究旨在探索氧化铁(Fe3O4)、金(Au)和银(Ag)等金属和金属氧化物纳米粒子(NPs)的抗氧化能力,以减轻人类红细胞中与年龄相关的氧化应激。金属和金属氧化物 NPs 的作用类似于抗氧化酶,可直接影响氧化还原途径,因此效率更高。此外,葡聚糖等生物聚合物涂层还能增强这些 NPs 的生物相容性。因此,我们采用湿化学方法合成了葡聚糖包覆的 Fe3O4、Au 和 Ag NPs,并对其进行了表征。研究了它们的血液相容性以及保护红细胞免受年龄诱导的氧化应激的能力。结果表明,Fe3O4 和 Au NPs 能保护红细胞免受过氧化氢和年龄诱导的氧化损伤,包括抗氧化剂水平下降、抗氧化酶活性降低和氧化物种数量增加。用 NPs 进行预处理可保持红细胞的形态和膜完整性。然而,Ag NPs 在红细胞中诱导的氧化应激与过氧化氢相似。因此,葡聚糖包覆的 Fe3O4 和金纳米粒子有可能被用作抗氧化疗法,以对抗与年龄有关的氧化应激。
Comparative Study of Antioxidant Activity of Dextran-Coated Iron Oxide, Gold, and Silver Nanoparticles Against Age-Induced Oxidative Stress in Erythrocytes.
Erythrocytes undergo several changes during human aging and age-related diseases and, thus, have been studied as biomarkers of the aging process. The present study aimed to explore the antioxidant ability of metal and metal oxide nanoparticles (NPs) such as iron oxide (Fe3O4), gold (Au), and silver (Ag) to mitigate age-related oxidative stress in human erythrocytes. Metal and metal oxide NPs behave like antioxidative enzymes, directly influencing redox pathways and thus have better efficiency. Additionally, biopolymer coatings such as dextran enhance the biocompatibility of these NPs. Therefore, dextran-coated Fe3O4, Au, and Ag NPs were synthesized using wet chemical methods and were characterized. Their hemocompatibility and ability to protect erythrocytes from age-induced oxidative stress were investigated. The Fe3O4 and Au NPs were observed to protect erythrocytes from hydrogen peroxide and age-induced oxidative damage, including decreased antioxidant levels, reduced activity of antioxidative enzymes, and increased amounts of oxidative species. Pretreatment with NPs preserved the morphology and membrane integrity of the erythrocyte. However, Ag NPs induced oxidative stress in erythrocytes similar to hydrogen peroxide. Therefore, dextran-coated Fe3O4 and Au nanoparticles have the potential to be employed as antioxidant therapies against age-related oxidative stress.