Lrig1+ 细胞在肾脏修复中的再生作用

IF 10.3 1区 医学 Q1 UROLOGY & NEPHROLOGY Journal of The American Society of Nephrology Pub Date : 2024-08-09 DOI:10.1681/ASN.0000000000000462
Yura Lee, Kwang H Kim, Jihwan Park, Hyun Mi Kang, Sung-Hee Kim, Haengdueng Jeong, Buhyun Lee, Nakyum Lee, Yejin Cho, Gyeong Dae Kim, Seyoung Yu, Heon Yung Gee, Jinwoong Bok, Maxwell S Hamilton, Leslie Gewin, Bruce J Aronow, Kyung-Min Lim, Robert J Coffey, Ki Taek Nam
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引用次数: 0

摘要

背景:在对严重肾损伤做出反应时,肾上皮细胞在适应性强的常驻上皮细胞的驱动下显示出卓越的再生能力。迄今为止,人们普遍认为成人肾脏缺乏多能干细胞;因此,对负责修复近端肾小管损伤的细胞系还不完全了解。富亮氨酸重复序列和免疫球蛋白样结构域蛋白1表达细胞(Lrig1+细胞)已被确定为各种组织中的长寿命细胞,可诱导上皮组织修复。因此,我们假设 Lrig1+ 细胞参与肾脏发育和组织再生:方法:我们利用小鼠模型研究了 Lrig1+ 细胞在肾损伤中的作用。方法:我们利用小鼠模型研究了 Lrig1+ 细胞在肾脏损伤中的作用。在三种不同的肾损伤小鼠模型中,使用他莫昔芬诱导的基于Lrig1特异性Cre重组酶的系谱追踪技术,在体内检测了Lrig1+后代细胞在急性肾损伤修复中的功能。此外,我们还进行了单细胞RNA测序,以确定Lrig1+细胞的转录特征并追踪其后代:结果:Lrig1+细胞存在于肾脏发育过程中,并在成熟小鼠肾脏近端小管和集合管结构的形成过程中做出了贡献。在三维培养中,单个 Lrig1+ 细胞表现出持久的繁殖能力,并分化成近端小管和集合管系。这些Lrig1+近端肾小管细胞高度表达祖细胞样基因和静止相关基因,从而形成了具有成体肾脏再生潜能的新型细胞群。此外,这些长寿命的 Lrig1+ 细胞在小鼠三种急性肾损伤中扩增并修复了受损的近端肾小管:这些发现凸显了 Lrig1+ 细胞在肾脏再生中的关键作用。
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Regenerative Role of Lrig1+ Cells in Kidney Repair.
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来源期刊
Journal of The American Society of Nephrology
Journal of The American Society of Nephrology 医学-泌尿学与肾脏学
CiteScore
22.40
自引率
2.90%
发文量
492
审稿时长
3-8 weeks
期刊介绍: The Journal of the American Society of Nephrology (JASN) stands as the preeminent kidney journal globally, offering an exceptional synthesis of cutting-edge basic research, clinical epidemiology, meta-analysis, and relevant editorial content. Representing a comprehensive resource, JASN encompasses clinical research, editorials distilling key findings, perspectives, and timely reviews. Editorials are skillfully crafted to elucidate the essential insights of the parent article, while JASN actively encourages the submission of Letters to the Editor discussing recently published articles. The reviews featured in JASN are consistently erudite and comprehensive, providing thorough coverage of respective fields. Since its inception in July 1990, JASN has been a monthly publication. JASN publishes original research reports and editorial content across a spectrum of basic and clinical science relevant to the broad discipline of nephrology. Topics covered include renal cell biology, developmental biology of the kidney, genetics of kidney disease, cell and transport physiology, hemodynamics and vascular regulation, mechanisms of blood pressure regulation, renal immunology, kidney pathology, pathophysiology of kidney diseases, nephrolithiasis, clinical nephrology (including dialysis and transplantation), and hypertension. Furthermore, articles addressing healthcare policy and care delivery issues relevant to nephrology are warmly welcomed.
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