通过 PI3K-AKT-GLUT2 途径改善钝口鳊鱼(Megalobrama amblycephala)的胰岛素反应和葡萄糖转运,从而补充高碳水化合物饮食中的色氨酸。

IF 4.8 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Nutritional Biochemistry Pub Date : 2024-08-09 DOI:10.1016/j.jnutbio.2024.109715
Kang Xiao, Xiaoyan Jia, Wei Qiang, Le Chang, Wenbin Liu, Dingdong Zhang
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引用次数: 0

摘要

本实验的目的是阐明在高碳水化合物日粮喂养情况下补充色氨酸对代谢的影响,这对改进水产养殖中的喂养策略以改善鱼类的碳水化合物代谢非常重要。初始平均体重为 55.0±0.5 克的钝吻鳊幼鱼被分配到三种实验日粮中的一种:CN:碳水化合物含量为 30%(重量比)的普通日粮;HC:碳水化合物含量为 43%(重量比)的高碳水化合物日粮;HL:添加了 0.8% L-色氨酸(L-trp)的高碳水化合物日粮。这些日粮喂养了 8 周,并评估了日粮中碳水化合物和色氨酸含量的影响。使用血红素和伊红(H&E)以及油红 O 染色法进行的组织学分析表明,高碳水化合物摄入与肝细胞形态异常和肝脏脂质过度积累有关,而补充色氨酸后这些情况明显改善。与对照组相比,高碳水化合物饮食后血浆葡萄糖、胰高血糖素、AGEs(高级糖化终产物)、甘油三酯、总胆固醇明显升高,胰岛素和肝糖原明显下降。在 HL 组中,几乎所有指标都恢复到了正常水平。本研究初步表明,补充色氨酸可改善高碳水化合物饮食引起的该物种葡萄糖代谢失衡。转录组学研究表明,高碳水化合物条件下的糖代谢主要受 PI3K-AKT 信号通路调控。GLUT2的mRNA表达和蛋白水平也随这一途径的变化而变化,这表明在高碳水化合物饮食条件下,色氨酸的添加会持续激活这一途径,从而加速葡萄糖转运和胰岛素分泌。随后的 GTT 和 ITT 实验也证明,色氨酸能改善高碳水化合物饮食下钝口鳊鱼的葡萄糖耐受性和胰岛素耐受性。总之,这些研究结果阐明了色氨酸在高碳水化合物日粮条件下对 PI3K-AKT-GLUT2 通路的正向调节作用,为今后合理应用高碳水化合物日粮提供了理论依据。
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Tryptophan supplements in high-carbohydrate diets by improving insulin response and glucose transport through PI3K-AKT-GLUT2 pathways in blunt snout bream (Megalobrama amblycephala)

The aim of this experiment was to elucidate the metabolic ramifications of tryptophan supplementation in the context of high-carbohydrate diet-feeding, which is important for improving feeding strategies in aquaculture in order to improve fish carbohydrate metabolism. Juvenile blunt snout bream with an initial mean body mass of 55.0±0.5 g were allocated to consume one of three experimental diets: CN, a normal diet with carbohydrate content of 30% (w/w); HC, a diet with high carbohydrate content of 43% (w/w); and HL, a high-carbohydrate diet to which 0.8% L-tryptophan (L-trp) had been added. These diets were fed for 8 weeks, and the effects of the carbohydrate and tryptophan contents of the diets were assessed.

Histological analysis using Hematoxylin and Eosin (H&E) and Oil Red O staining revealed that high-carbohydrate intake was associated with abnormal hepatocyte morphology and excessive liver lipid accumulation, which were notably ameliorated by tryptophan supplementation. A significant increase in plasma glucose, glucagon, AGEs (advanced glycation end products), triglycerides, total cholesterol, and a significant decrease in insulin and hepatic glycogen after a high-carbohydrate diet in terms of plasma indices, compared to the control group. Almost all of them were restored to the normal level in the HL group. The present study might preliminarily suggest that tryptophan supplementation ameliorates the imbalance in glucose metabolism of this species induced by a high-carbohydrate diet. Transcriptomics showed that glucose metabolism under high carbohydrate was mainly regulated by the PI3K-AKT signaling pathway. The mRNA expression and protein levels of GLUT2 also varied with this pathway, which would suggest that sustained activation of this pathway with the addition of tryptophan accelerates glucose transport and insulin secretion under high-carbohydrate diet. Subsequent GTT and ITT experiments have also demonstrated that tryptophan improves glucose tolerance and insulin tolerance in blunt snout bream on a high-carbohydrate diet.

In conclusion, these findings elucidate the positive regulatory effect of tryptophan on the PI3K-AKT-GLUT2 pathway under a high carbohydrate diet and provide a theoretical basis for the subsequent rational application of high carbohydrate diets in the future.

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来源期刊
Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry 医学-生化与分子生物学
CiteScore
9.50
自引率
3.60%
发文量
237
审稿时长
68 days
期刊介绍: Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
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