不同的肠型和菌群失调:揭开肺病和重症医学住院病人肠道微生物群的神秘面纱。

IF 5.8 2区 医学 Q1 Medicine Respiratory Research Pub Date : 2024-08-10 DOI:10.1186/s12931-024-02943-7
Naijian Li, Guiyan Tan, Zhiling Xie, Weixin Chen, Zhaowei Yang, Zhang Wang, Sha Liu, Mengzhang He
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引用次数: 0

摘要

背景:肠道-肺轴对呼吸系统健康至关重要,但对肺部和重症监护医学(PCCM)住院患者的肠道-肺轴研究不足:方法:我们对三所医科大学附属医院的 PCCM 住院患者的粪便样本(住院患者,n = 374;健康对照组,n = 105)进行了 16S 核糖体 RNA 测序。我们对 PCCM 住院患者的肠道微生物群组成进行了统计分析,并与健康对照组进行了比较。此外,我们还探讨了肠道微生物群组成与各种临床因素(包括年龄、白细胞计数、中性粒细胞计数、血小板计数、白蛋白水平、血红蛋白水平、住院时间和医疗费用)之间的关联:与健康对照组相比,PCCM 住院患者的肠道微生物群多样性较低。主坐标分析显示,微生物群的整体结构存在明显差异。确定了四种肠型,包括住院患者独有的肠球菌肠型。虽然在门一级没有发现差异,但有 15 个细菌科表现出不同的丰度。具体而言,来自 PCCM 的住院病人中肠球菌科、乳酸菌科、鞘氨醇梭菌科、梭菌科和丹拿菌科细菌的数量明显较多。通过随机森林分析,我们计算出区分健康人和住院病人的接收者操作特征曲线下面积(AUC)为 0.75(95% CIs 0.69-0.80)。分类器中保留最多的四个菌属是布劳氏菌、亚多利格兰菌、肠球菌和克雷伯氏菌:结论:PCCM 住院患者肠道微生物群失调的证据凸显了肠道-肺轴的重要性,有望为呼吸健康研究提供新的途径。
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Distinct enterotypes and dysbiosis: unraveling gut microbiota in pulmonary and critical care medicine inpatients.

Background: The gut-lung axis, pivotal for respiratory health, is inadequately explored in pulmonary and critical care medicine (PCCM) inpatients.

Methods: Examining PCCM inpatients from three medical university-affiliated hospitals, we conducted 16S ribosomal RNA sequencing on stool samples (inpatients, n = 374; healthy controls, n = 105). We conducted statistical analyses to examine the gut microbiota composition in PCCM inpatients, comparing it to that of healthy controls. Additionally, we explored the associations between gut microbiota composition and various clinical factors, including age, white blood cell count, neutrophil count, platelet count, albumin level, hemoglobin level, length of hospital stay, and medical costs.

Results: PCCM inpatients exhibited lower gut microbiota diversity than healthy controls. Principal Coordinates Analysis revealed marked overall microbiota structure differences. Four enterotypes, including the exclusive Enterococcaceae enterotype in inpatients, were identified. Although no distinctions were found at the phylum level, 15 bacterial families exhibited varying abundances. Specifically, the inpatient population from PCCM showed a significantly higher abundance of Enterococcaceae, Lactobacillaceae, Erysipelatoclostridiaceae, Clostridiaceae, and Tannerellaceae. Using random forest analyses, we calculated the areas under the receiver operating characteristic curves (AUCs) to be 0.75 (95% CIs 0.69-0.80) for distinguishing healthy individuals from inpatients. The four most abundant genera retained in the classifier were Blautia, Subdoligranulum, Enterococcus, and Klebsiella.

Conclusions: Evidence of gut microbiota dysbiosis in PCCM inpatients underscores the gut-lung axis's significance, promising further avenues in respiratory health research.

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来源期刊
Respiratory Research
Respiratory Research RESPIRATORY SYSTEM-
CiteScore
9.70
自引率
1.70%
发文量
314
审稿时长
4-8 weeks
期刊介绍: Respiratory Research publishes high-quality clinical and basic research, review and commentary articles on all aspects of respiratory medicine and related diseases. As the leading fully open access journal in the field, Respiratory Research provides an essential resource for pulmonologists, allergists, immunologists and other physicians, researchers, healthcare workers and medical students with worldwide dissemination of articles resulting in high visibility and generating international discussion. Topics of specific interest include asthma, chronic obstructive pulmonary disease, cystic fibrosis, genetics, infectious diseases, interstitial lung diseases, lung development, lung tumors, occupational and environmental factors, pulmonary circulation, pulmonary pharmacology and therapeutics, respiratory immunology, respiratory physiology, and sleep-related respiratory problems.
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