新月酵母菌对钾吸收的调节。

IF 2.7 3区 生物学 Q3 MICROBIOLOGY Journal of Bacteriology Pub Date : 2024-09-19 Epub Date: 2024-08-12 DOI:10.1128/jb.00107-24
Alex Quintero-Yanes, Loïc Léger, Madeline Collignon, Julien Mignon, Aurélie Mayard, Catherine Michaux, Régis Hallez
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引用次数: 0

摘要

钾(K+)是决定细胞膜电位、细胞内 pH 值、渗透压/压力和蛋白质合成的重要生理元素。在这里,我们描述了作为不对称细胞分裂模型的寡营养型α-新月菌(Caulobacter crescentus)中钾摄取系统的调控。我们的研究表明,新月菌主要利用两种钾转运体(低亲和力的 Kup 和高亲和力的 Kdp 摄取系统)来维持钾的平衡,从而能在微摩尔到毫摩尔浓度范围内生长。当 K+ 不受限制时,我们发现 kup 基因是必不可少的,而 kdp 失活不会影响生长。与此相反,在 K+ 有限的环境中,kdp 对生长变得关键但不是必需的,而 kup 则是可有可无的。然而,在没有 kdp 的情况下,kup 的突变被选中来改善 K+ 贫乏条件下的生长,这可能是通过增加 Kup 对 K+ 的亲和力实现的。此外,调控 kdpABCDE 表达的 KdpDE 双组分系统的突变表明,内膜传感器调控组分 KdpD 主要作为磷酸酶发挥作用,在细胞进入指数期后期限制生长。因此,我们的数据表明,KdpE 被另一种非认知组氨酸激酶磷酸化。此外,我们还测定了依赖于 KdpE 和独立于 K+ 的转录组。总之,我们的工作说明了寡营养细菌是如何对 K+ 可用性的波动做出反应的。重要意义钾(K+)是一种关键的金属离子,参与了许多重要的细胞过程。在这里,我们展示了新月芽孢杆菌(Caulobacter crescentus)在微摩尔浓度的 K+ 条件下主要通过两种 K+ 吸收系统(低亲和力 Kup 和高亲和力 Kdp)来支持生长。利用全基因组方法,我们还确定了新月杆菌在低 K+浓度下存活所需的全部基因集以及全部 K+依赖性调控子。最后,我们发现由 KdpDE 双组分系统介导的转录调控是非常规的,因为与大肠杆菌不同,内膜传感器调控组分 KdpD 似乎是作为磷酸化反应调控因子 KdpE~P 的磷酸酶起作用。
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Regulation of potassium uptake in Caulobacter crescentus.

Potassium (K+) is an essential physiological element determining membrane potential, intracellular pH, osmotic/turgor pressure, and protein synthesis in cells. Here, we describe the regulation of potassium uptake systems in the oligotrophic α-proteobacterium Caulobacter crescentus known as a model for asymmetric cell division. We show that C. crescentus can grow in concentrations from the micromolar to the millimolar range by mainly using two K+ transporters to maintain potassium homeostasis, the low-affinity Kup and the high-affinity Kdp uptake systems. When K+ is not limiting, we found that the kup gene is essential while kdp inactivation does not impact the growth. In contrast, kdp becomes critical but not essential and kup dispensable for growth in K+-limited environments. However, in the absence of kdp, mutations in kup were selected to improve growth in K+-depleted conditions, likely by increasing the affinity of Kup for K+. In addition, mutations in the KdpDE two-component system, which regulates kdpABCDE expression, suggest that the inner membrane sensor regulatory component KdpD mainly works as a phosphatase to limit the growth when cells reach late exponential phase. Our data therefore suggest that KdpE is phosphorylated by another non-cognate histidine kinase. On top of this, we determined the KdpE-dependent and independent K+ transcriptome. Together, our work illustrates how an oligotrophic bacterium responds to fluctuation in K+ availability.IMPORTANCEPotassium (K+) is a key metal ion involved in many essential cellular processes. Here, we show that the oligotroph Caulobacter crescentus can support growth at micromolar concentrations of K+ by mainly using two K+ uptake systems, the low-affinity Kup and the high-affinity Kdp. Using genome-wide approaches, we also determined the entire set of genes required for C. crescentus to survive at low K+ concentration as well as the full K+-dependent regulon. Finally, we found that the transcriptional regulation mediated by the KdpDE two-component system is unconventional since unlike Escherichia coli, the inner membrane sensor regulatory component KdpD seems to work rather as a phosphatase on the phosphorylated response regulator KdpE~P.

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来源期刊
Journal of Bacteriology
Journal of Bacteriology 生物-微生物学
CiteScore
6.10
自引率
9.40%
发文量
324
审稿时长
1.3 months
期刊介绍: The Journal of Bacteriology (JB) publishes research articles that probe fundamental processes in bacteria, archaea and their viruses, and the molecular mechanisms by which they interact with each other and with their hosts and their environments.
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