四种梅属植物赤霉素氧化酶家族成员的全基因组分析以及 PmGA2ox8 对植物高度的功能分析

IF 4.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY International Journal of Molecular Sciences Pub Date : 2024-08-09 DOI:10.3390/ijms25168697
Xue Li, Jie Zhang, Xiaoyu Guo, Like Qiu, Ke Chen, Jia Wang, T. Cheng, Qixiang Zhang, Tangchun Zheng
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引用次数: 0

摘要

赤霉素(GA)是一种在植物生长发育过程中起重要作用的酶,其在植物体内的含量可由赤霉素氧化酶(GAoxs)调节。作为重要的果树和观赏植物,研究梅属植物结构形成的机制至关重要。在此,研究人员从梅花属、杏属、樱花属和柿属植物中鉴定出 85 个 GAox 基因,并将它们分为六个亚群。保守基序和基因结构分析表明,GAoxs 在四个梅科植物中是保守的。共线性分析表明,梅基因组中有两个PmGAoxs片段复制事件。启动子顺式元件分析显示,24个PmGAoxs含有激素反应元件和发育调控元件。表达谱表明 PmGAoxs 具有组织表达特异性,花芽休眠期的 GA 水平受某些 PmGAoxs 控制。经 IAA 或 GA3 处理后,PmGA2ox8 在茎中的转录水平显著增加,并在直立茎和垂枝茎中表现出不同的表达水平。在拟南芥的根、茎、叶和花器官中都检测到了由 PmGA2ox8 启动子驱动的 GUS 活性。PmGA2ox8在拟南芥中的过表达导致矮化表型、莲座叶数量增加但叶面积减少以及开花延迟。我们的研究结果表明,GAoxs在拟南芥物种中是保守的,而PmGA2ox8在调节植株高度中起着重要作用。
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Genome-Wide Analysis of the Gibberellin-Oxidases Family Members in Four Prunus Species and a Functional Analysis of PmGA2ox8 in Plant Height
Gibberellins (GAs), enzymes that play a significant role in plant growth and development, and their levels in plants could be regulated by gibberellin-oxidases (GAoxs). As important fruit trees and ornamental plants, the study of the mechanism of plant architecture formation of the Prunus genus is crucial. Here, 85 GAox genes were identified from P. mume, P. armeniaca, P. salicina, and P. persica, and they were classified into six subgroups. Conserved motif and gene structure analysis showed that GAoxs were conserved in the four Prunus species. Collinearity analysis revealed two fragment replication events of PmGAoxs in the P. mume genome. Promoter cis-elements analysis revealed 24 PmGAoxs contained hormone-responsive elements and development regulatory elements. The expression profile indicated that PmGAoxs have tissue expression specificity, and GA levels during the dormancy stage of flower buds were controlled by certain PmGAoxs. After being treated with IAA or GA3, the transcription level of PmGA2ox8 in stems was significantly increased and showed a differential expression level between upright and weeping stems. GUS activity driven by PmGA2ox8 promoter was detected in roots, stems, leaves, and flower organs of Arabidopsis. PmGA2ox8 overexpression in Arabidopsis leads to dwarfing phenotype, increased number of rosette leaves but decreased leaf area, and delayed flowering. Our results showed that GAoxs were conserved in Prunus species, and PmGA2ox8 played an essential role in regulating plant height.
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来源期刊
International Journal of Molecular Sciences
International Journal of Molecular Sciences Chemistry-Organic Chemistry
CiteScore
8.10
自引率
10.70%
发文量
13472
审稿时长
17.49 days
期刊介绍: The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).
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