不同信号肽在提高重组蛋白在大肠杆菌中溶解度方面的研究：微型综述文章

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS Current Proteomics Pub Date : 2024-08-07 DOI:10.2174/0115701646305738240730113619

Seyyed Soheil Rahmatabadi, Soudabeh Askari, Fatemeh Khademi, Bijan Soleymani

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引用次数: 0

摘要

在大肠杆菌中，以包涵体（IBs）形式生产蛋白质会降低这些产物的溶解度和活性。为了克服这一问题，研究人员采用了多种方法。通过合适的信号肽将重组蛋白分泌到外质空间是解决以原生形式生产重组蛋白的局限性的一种方法。在细菌宿主中分泌性生产重组蛋白有很多优点，因此是一个令人感兴趣的话题。然而，由于工艺难度大，而且需要针对不同宿主和蛋白质选择合适的信号肽，因此很难实现。根据文献，实验中应用了不同的信号肽来提高各种重组蛋白的溶解度。实验表明，特定蛋白质的分泌效率会因蛋白质上所附信号肽的类型而大不相同。因此，信号肽的选择和改变是本综述讨论的改善重组蛋白分泌的两个关键方法。此外，还讨论了影响重组蛋白表达和溶解度的不同因素。

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The Study of Different Signal Peptides in Improvement of Recombinant Proteins Solubility in E. coli: A Mini-Review Article

In E. coli, the production of proteins as inclusion bodies (IBs) caused a decrease in the solubility and activity of these products. Diverse approaches and methods have been used by investigators to overcome this problem. The secretion of recombinant proteins into the periplasmic space by means of suitable signal peptides is a way to resolve these limitations for the production of recombinant proteins in a native form. Secretory production of recombinant proteins in bacterial hosts has many advantages and thus, it is a topic of interest. However, it is hard to achieve due to the difficulty of the process and the need for the choice of appropriate signal peptide for each host and protein. Based on the literature, different signal peptides have experimentally been applied to enhance the solubility of various recombinant proteins. It has been shown that the secretion efficiency of a given protein differs dramatically based on the type of the signal peptide that is attached to the protein. Therefore, the choice and alteration of signal peptides are the two crucial approaches for the improvement of a recombinant protein secretion that have been discussed in this review. Also, different factors affecting the expression and solubility of recombinant proteins have been discussed.

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.