Ling Li, Yueyan Wang, Lang Gao, Shunying Wu, Ying Jin
{"title":"姜黄素碳点通过调节 METTL3/IRE1α 信号抑制牙周炎","authors":"Ling Li, Yueyan Wang, Lang Gao, Shunying Wu, Ying Jin","doi":"10.1615/critreveukaryotgeneexpr.2024054463","DOIUrl":null,"url":null,"abstract":"This study aimed to investigate the effects of curcumin-carbon dot conjugates (CUR-CD) on periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS) was used to establish periodontitis mode in vivo and in vitro. Histological analysis was conducted using HE staining. BMP2 and RUNX2 expression was determined using immunohistochemistry. mRNA levels were detected using RT-qPCR. Cytokine release was determined using ELISA assay. Osteogenic differentiation was detected using ALP staining. The results showed that CUR-CD inhibited the inflammatory response, as well as promoted bone healing in vivo and osteogenic differentiation in vitro. Moreover, CUR-CD downregulated METTL3, which inhibited m6A modification of IRE1α and downregulated IRE1α expression. However, overexpression of IRE1α reversed the effects of CUR-CD, stimulating inflammatory response and inhibiting bone healing and osteogenic differentiation. Collectively, CUR-CD inhibits the progression of periodontitis via downregulating IRE1α. Therefore, CUR-CD may be an alternative strategy for periodontitis.","PeriodicalId":56317,"journal":{"name":"Critical Reviews in Eukaryotic Gene Expression","volume":"20 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Curcumin-carbon dots suppress periodontitis via regulating METTL3/IRE1α signaling\",\"authors\":\"Ling Li, Yueyan Wang, Lang Gao, Shunying Wu, Ying Jin\",\"doi\":\"10.1615/critreveukaryotgeneexpr.2024054463\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aimed to investigate the effects of curcumin-carbon dot conjugates (CUR-CD) on periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS) was used to establish periodontitis mode in vivo and in vitro. Histological analysis was conducted using HE staining. BMP2 and RUNX2 expression was determined using immunohistochemistry. mRNA levels were detected using RT-qPCR. Cytokine release was determined using ELISA assay. Osteogenic differentiation was detected using ALP staining. The results showed that CUR-CD inhibited the inflammatory response, as well as promoted bone healing in vivo and osteogenic differentiation in vitro. Moreover, CUR-CD downregulated METTL3, which inhibited m6A modification of IRE1α and downregulated IRE1α expression. However, overexpression of IRE1α reversed the effects of CUR-CD, stimulating inflammatory response and inhibiting bone healing and osteogenic differentiation. Collectively, CUR-CD inhibits the progression of periodontitis via downregulating IRE1α. Therefore, CUR-CD may be an alternative strategy for periodontitis.\",\"PeriodicalId\":56317,\"journal\":{\"name\":\"Critical Reviews in Eukaryotic Gene Expression\",\"volume\":\"20 1\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Critical Reviews in Eukaryotic Gene Expression\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054463\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Critical Reviews in Eukaryotic Gene Expression","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1615/critreveukaryotgeneexpr.2024054463","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Curcumin-carbon dots suppress periodontitis via regulating METTL3/IRE1α signaling
This study aimed to investigate the effects of curcumin-carbon dot conjugates (CUR-CD) on periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS) was used to establish periodontitis mode in vivo and in vitro. Histological analysis was conducted using HE staining. BMP2 and RUNX2 expression was determined using immunohistochemistry. mRNA levels were detected using RT-qPCR. Cytokine release was determined using ELISA assay. Osteogenic differentiation was detected using ALP staining. The results showed that CUR-CD inhibited the inflammatory response, as well as promoted bone healing in vivo and osteogenic differentiation in vitro. Moreover, CUR-CD downregulated METTL3, which inhibited m6A modification of IRE1α and downregulated IRE1α expression. However, overexpression of IRE1α reversed the effects of CUR-CD, stimulating inflammatory response and inhibiting bone healing and osteogenic differentiation. Collectively, CUR-CD inhibits the progression of periodontitis via downregulating IRE1α. Therefore, CUR-CD may be an alternative strategy for periodontitis.
期刊介绍:
Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource.
Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.