开发和应用胶体金试纸快速检测犬布氏杆菌病

Biosensors Pub Date : 2024-08-10 DOI:10.3390/bios14080388
Pengxiang Sun, Xinmei Yang, Jinyue Liu, Yanqing Bao, Jingjing Qi, Xiangan Han, Guanhui Liu, Shaohui Wang, Mingxing Tian
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引用次数: 0

摘要

布鲁氏菌病是一个全球性问题,病原体是布鲁氏菌属。犬布鲁氏菌可引起犬的波状热,这是一种人畜共患病,不仅可在犬之间传播,还可传染给人类。这对社会构成了公共卫生威胁。本研究开发了一种快速、简便的免疫胶体金试纸,通过检测血清样本中的抗 LPS 抗体来诊断犬布鲁氏杆菌病。胶体金标记的抗体为与胶体金共轭的兔抗犬 IgG。检测线(T 线)以提取的高纯度 R-LPS 作为捕获抗原,对照线(C 线)以山羊抗兔 IgG 作为捕获抗体。胶体金试纸条在检测布鲁氏菌病方面具有高度特异性,与犬冠状病毒(CCV)、犬瘟热病毒(CDV)和犬细小病毒(CPV)引起的临床常见犬病没有交叉反应。与市售的 iELISA 试剂盒相比,胶体金试纸的灵敏度和特异度分别为 95.23% 和 98.76%。诊断吻合率为 98.47%。这项研究结果表明,胶体金试纸可作为一种直接、快速、灵敏和特异的诊断工具,用于犬布鲁氏杆菌病的鉴定,尤其是在资源有限的地区。
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Development and Application of Colloidal Gold Test Strips for the Rapid Detection of Canine Brucellosis
Brucellosis is a global problem, with the causative agent being the genus Brucella. B. canis can cause undulant fever in dogs, which is a zoonotic disease that can spread not only among dogs but also to humans. This poses a public health threat to society. In this study, a rapid and straightforward immune colloidal gold test strip was developed for the diagnosis of canine brucellosis through the detection of anti-LPS antibodies in serum samples. Rabbit anti-canine IgG conjugated with colloidal gold was employed as the colloidal gold-labeled antibody. The extracted high-purity R-LPS was employed as the capture antigen in the test line (T-line), while goat anti-rabbit IgG was utilized as the capture antibody in the control line (C-line). The colloidal gold strip exhibited high specificity in the detection of brucellosis, with no cross-reaction observed with the common clinical canine diseases caused by Canine coronavirus (CCV), Canine distemper virus (CDV), and Canine parvovirus (CPV). In comparison to the commercial iELISA kit, the sensitivity and specificity of the colloidal gold test strip were found to be 95.23% and 98.76%, respectively. The diagnostic coincidence rate was 98.47%. The findings of this study indicate that colloidal gold test strips may be employed as a straightforward, expeditious, sensitive, and specific diagnostic instrument for the identification of canine brucellosis, particularly in resource-limited regions.
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