{"title":"约旦绵羊和山羊奶中李斯特菌的分子血清分组和毒力","authors":"","doi":"10.1016/j.idairyj.2024.106051","DOIUrl":null,"url":null,"abstract":"<div><p><em>Listeria monocytogenes</em> is a serious pathogen with several serogroups that differ in virulence and distribution among countries. This study determined the serogroups and virulence of 116 <em>L</em>. <em>monocytogenes</em> from sheep milk (n = 52) and goat milk (n = 64) in Jordan by several multiplex PCR. The results showed that the <em>L</em>. <em>monocytogenes</em> isolates belong to five serogroups. Most isolates (37.9%) belonged to serogroup 4b (d, e), followed by serogroup 1/2a (3a) (31.9%), serogroup 1/2b (26.7%), and serogroup 4a/c (2.6%) and serogroup IV (serotypes 1/2c and 3c) (0.9%). All isolates carried the internalin (<em>inlA</em>, <em>inlC</em>, <em>inlJ</em>) and <em>lmo</em> 2672 genes. Additionally, 36.4% and 29.0 % of 4b and 1/2b isolates carried the Listeriolysin S gene (<em>lls</em>X). Therefore, <em>L</em>. <em>monocytogenes</em> in sheep and goat milk are potential public health hazards because they belong to the major listeriosis-causing serogroups (4b, 1/2a, and 1/2b), and carry major virulence genes. Small ruminants’ milk and cheeses should be considered high-risk foods for <em>L</em>. <em>monocytogenes</em> infection. Thus, safe production practices should be monitored and enforced in small dairy shops.</p></div>","PeriodicalId":13854,"journal":{"name":"International Dairy Journal","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular serogrouping and virulence of Listeria monocytogenes from sheep and goat milk in Jordan\",\"authors\":\"\",\"doi\":\"10.1016/j.idairyj.2024.106051\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Listeria monocytogenes</em> is a serious pathogen with several serogroups that differ in virulence and distribution among countries. This study determined the serogroups and virulence of 116 <em>L</em>. <em>monocytogenes</em> from sheep milk (n = 52) and goat milk (n = 64) in Jordan by several multiplex PCR. The results showed that the <em>L</em>. <em>monocytogenes</em> isolates belong to five serogroups. Most isolates (37.9%) belonged to serogroup 4b (d, e), followed by serogroup 1/2a (3a) (31.9%), serogroup 1/2b (26.7%), and serogroup 4a/c (2.6%) and serogroup IV (serotypes 1/2c and 3c) (0.9%). All isolates carried the internalin (<em>inlA</em>, <em>inlC</em>, <em>inlJ</em>) and <em>lmo</em> 2672 genes. Additionally, 36.4% and 29.0 % of 4b and 1/2b isolates carried the Listeriolysin S gene (<em>lls</em>X). Therefore, <em>L</em>. <em>monocytogenes</em> in sheep and goat milk are potential public health hazards because they belong to the major listeriosis-causing serogroups (4b, 1/2a, and 1/2b), and carry major virulence genes. Small ruminants’ milk and cheeses should be considered high-risk foods for <em>L</em>. <em>monocytogenes</em> infection. Thus, safe production practices should be monitored and enforced in small dairy shops.</p></div>\",\"PeriodicalId\":13854,\"journal\":{\"name\":\"International Dairy Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-07-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Dairy Journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0958694624001717\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Dairy Journal","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0958694624001717","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Molecular serogrouping and virulence of Listeria monocytogenes from sheep and goat milk in Jordan
Listeria monocytogenes is a serious pathogen with several serogroups that differ in virulence and distribution among countries. This study determined the serogroups and virulence of 116 L. monocytogenes from sheep milk (n = 52) and goat milk (n = 64) in Jordan by several multiplex PCR. The results showed that the L. monocytogenes isolates belong to five serogroups. Most isolates (37.9%) belonged to serogroup 4b (d, e), followed by serogroup 1/2a (3a) (31.9%), serogroup 1/2b (26.7%), and serogroup 4a/c (2.6%) and serogroup IV (serotypes 1/2c and 3c) (0.9%). All isolates carried the internalin (inlA, inlC, inlJ) and lmo 2672 genes. Additionally, 36.4% and 29.0 % of 4b and 1/2b isolates carried the Listeriolysin S gene (llsX). Therefore, L. monocytogenes in sheep and goat milk are potential public health hazards because they belong to the major listeriosis-causing serogroups (4b, 1/2a, and 1/2b), and carry major virulence genes. Small ruminants’ milk and cheeses should be considered high-risk foods for L. monocytogenes infection. Thus, safe production practices should be monitored and enforced in small dairy shops.
期刊介绍:
The International Dairy Journal publishes significant advancements in dairy science and technology in the form of research articles and critical reviews that are of relevance to the broader international dairy community. Within this scope, research on the science and technology of milk and dairy products and the nutritional and health aspects of dairy foods are included; the journal pays particular attention to applied research and its interface with the dairy industry.
The journal''s coverage includes the following, where directly applicable to dairy science and technology:
• Chemistry and physico-chemical properties of milk constituents
• Microbiology, food safety, enzymology, biotechnology
• Processing and engineering
• Emulsion science, food structure, and texture
• Raw material quality and effect on relevant products
• Flavour and off-flavour development
• Technological functionality and applications of dairy ingredients
• Sensory and consumer sciences
• Nutrition and substantiation of human health implications of milk components or dairy products
International Dairy Journal does not publish papers related to milk production, animal health and other aspects of on-farm milk production unless there is a clear relationship to dairy technology, human health or final product quality.