有机硒导尿管材料完全抑制铜绿假单胞菌的生长

Phat L. Tran, Caroline L. Presson, Md Nayeem Hasan Kashem, Wei Li, Ted W. Reid, Werner T. W. de Riese
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引用次数: 0

摘要

为了进一步研究有机硒结合聚氨酯(PU)导管材料对铜绿假单胞菌体外生长和生物膜形成的抑制作用。在谷胱甘肽存在的情况下,将铜绿假单胞菌、金黄色葡萄球菌和白色念珠菌与有机硒和对照聚氨酯导管材料进行体外培养。生长情况通过菌落形成单位(CFU)计数进行评估,并用激光共聚焦扫描显微镜进行观察。使用了两种不同的聚氨酯导管材料。使用锡催化聚氨酯导管材料,硒(Se)含量为 1%时可完全抑制金黄色葡萄球菌的生长,而硒含量高达 3.0%时则不能抑制铜绿假单胞菌的生长。而使用热塑性聚氨酯导管材料时,1.5%Se 和 2%Se 的有机硒对铜绿假单胞菌的生长有几个对数的抑制作用,2.5% 的硒则有完全抑制作用(8 个对数)。硒含量低于 1.5%的样品对铜绿假单胞菌的生长抑制作用不明显。对耐多种药物的白细胞菌株也有类似的体外生长抑制作用。结论是,在热塑性聚氨酯导管材料中加入 2.5% 的硒作为有机硒,可对铜绿假单胞菌的体外生长和生物膜形成产生最佳抑制作用。这些结果表明,使用有机硒导管可降低铜绿假单胞菌及其他细菌和真菌的 CAUTI(导管相关性尿路感染)发病率。
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Complete Growth Inhibition of Pseudomonas aeruginosa by Organo-Selenium-Incorporated Urinary Catheter Material
To further investigate the inhibition of Pseudomonas aeruginosa’s in vitro growth and biofilm formation by an organo-selenium-incorporated polyurethane (PU) catheter material. P. aeruginosa, Staphylococcus aureus, and Candida albicans were incubated in vitro with organo-selenium and control polyurethane catheter materials in the presence of glutathione. Growth was evaluated by a colony-forming-unit (CFU) count and visualized with confocal laser scanning microscopy. Two different PU catheter materials were used. Using tin-catalyzed PU catheter material, complete inhibition of S. aureus was seen at 1% selenium (Se), whereas no inhibition was seen for P. aeruginosa at up to 3.0% Se. Whereas, using a thermoplastic PU catheter material, 1.5% Se and 2% Se organo-selenium caused several logs of growth inhibition of P. aeruginosa, and 2.5% selenium, incorporation showed complete inhibition (8 logs). Samples with lower than 1.5% selenium did not show adequate growth inhibition for P. aeruginosa. Similar in vitro growth inhibition was achieved against a multidrug-resistant C. albicans strain. It was concluded that optimal inhibition of P. aeruginosa in vitro growth and biofilm formation occurs with 2.5% selenium incorporated as organo-selenium in a thermoplastic PU catheter material. These results suggest that reduced incidence of CAUTIs (catheter associated urinary tract infections) with P. aeruginosa and other bacteria and fungi can be achieved by using organo-selenium-incorporated catheters.
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