檀香(Santalum album L.)叶绿体基因组的长线程测序分析

AMM Aryana, M Majiidu, R Pratama, FG Dwiyanti and IZ Siregar
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引用次数: 0

摘要

檀香(Santalum album L.)是一种以生产具有高经济价值的精油而闻名的树种。然而,由于其自然种群中存在不可持续的管理方法,檀香的潜力面临着巨大挑战。基因技术是缓解檀香树可持续性挑战的可行解决方案,为檀香树的保护和长期管理提供了至关重要的见解。因此,人们认识到有必要提供大戟科植物的叶绿体基因组信息,从而扩充现有的基因数据库,促进制定有效的保护策略,实现该物种的可持续发展。本研究旨在利用牛津纳米孔技术公司(Oxford Nanopore Technologies)的 MinION 获得长读数序列数据,生成白相思树叶绿体基因组,并通过系统发生树分析白相思树与其他物种的遗传关系。这项研究成功生成了一个完整的白相思树叶绿体基因组,由143.261个碱基对组成,有望用于各种遗传分析应用。这些应用为未来相思鸟的保护带来了希望,为可持续管理和保护提供了新的途径。
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Long-read sequencing analysis of the chloroplast genome in sandalwood (Santalum album L.)
Santalum album L. (sandalwood) is a tree species renowned for producing essential oil with high economic value. However, the potential of S. album faces significant challenges due to unsustainable management practices within its natural population. Genetic techniques present a viable solution to mitigating S. album sustainability challenges, offering insights crucial for its conservation and long-term management. Hence, there is a recognised necessity to furnish chloroplast genome information for S. album, thereby augmenting the existing genetic database and facilitating the formulation of effective conservation strategies for the sustainability of this species. This research aims to obtain the long-read sequence data using MinION from Oxford Nanopore Technologies for generating the S. album chloroplast genome, and to analyse genetic relationships of S. album with other species through a phylogenetic tree. The study successfully generated a completed chloroplast genome of S. album, consisting of 143.261 base pairs, which has the potential to be used in various applications of genetic analysis. These applications hold promise for the future of S. album conservation, offering new avenues for sustainable management and preservation.
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