{"title":"β-半乳糖苷酶:洞察来源变异、基因工程、固定化以及在食品、工业和医药领域的多种应用","authors":"Yang Zhou, Yuelin Liu, Fukang Gao, Zhenzhu Xia, Zhoufan Zhang, Frank Peprah Addai, Yiyin Zhu, Jinping Chen, Feng Lin, Dongfeng Chen","doi":"10.1111/1471-0307.13098","DOIUrl":null,"url":null,"abstract":"<p>β-Galactosidases are crucial enzymes that hydrolyse oligosaccharides and polysaccharides with terminal β-1,4-glycosidic bonds. Though the traditional application of β-Galactosidases has been to catalyse the breakdown of lactose in dairy products, its application extends beyond the production of lactose-free products since variants capable of facilitating lactose condensation and exhibiting galactosyl transferase activity are extensively utilised for the synthesis of prebiotic galacto-oligosaccharides. This review analyses β-Galactosidase in multiple aspects, including sources, classification, characterisation, immobilisation, genetic engineering and applications in terms of whey treatment, biofuel production, production of lactose-free dietary product, synthesis of galacto-oligosaccharides and the early detection of cellular senescence and tumours.</p>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"β-Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine\",\"authors\":\"Yang Zhou, Yuelin Liu, Fukang Gao, Zhenzhu Xia, Zhoufan Zhang, Frank Peprah Addai, Yiyin Zhu, Jinping Chen, Feng Lin, Dongfeng Chen\",\"doi\":\"10.1111/1471-0307.13098\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>β-Galactosidases are crucial enzymes that hydrolyse oligosaccharides and polysaccharides with terminal β-1,4-glycosidic bonds. Though the traditional application of β-Galactosidases has been to catalyse the breakdown of lactose in dairy products, its application extends beyond the production of lactose-free products since variants capable of facilitating lactose condensation and exhibiting galactosyl transferase activity are extensively utilised for the synthesis of prebiotic galacto-oligosaccharides. This review analyses β-Galactosidase in multiple aspects, including sources, classification, characterisation, immobilisation, genetic engineering and applications in terms of whey treatment, biofuel production, production of lactose-free dietary product, synthesis of galacto-oligosaccharides and the early detection of cellular senescence and tumours.</p>\",\"PeriodicalId\":13822,\"journal\":{\"name\":\"International Journal of Dairy Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Dairy Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1471-0307.13098\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Dairy Technology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1471-0307.13098","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
β-Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine
β-Galactosidases are crucial enzymes that hydrolyse oligosaccharides and polysaccharides with terminal β-1,4-glycosidic bonds. Though the traditional application of β-Galactosidases has been to catalyse the breakdown of lactose in dairy products, its application extends beyond the production of lactose-free products since variants capable of facilitating lactose condensation and exhibiting galactosyl transferase activity are extensively utilised for the synthesis of prebiotic galacto-oligosaccharides. This review analyses β-Galactosidase in multiple aspects, including sources, classification, characterisation, immobilisation, genetic engineering and applications in terms of whey treatment, biofuel production, production of lactose-free dietary product, synthesis of galacto-oligosaccharides and the early detection of cellular senescence and tumours.
期刊介绍:
The International Journal of Dairy Technology ranks highly among the leading dairy journals published worldwide, and is the flagship of the Society. As indicated in its title, the journal is international in scope.
Published quarterly, International Journal of Dairy Technology contains original papers and review articles covering topics that are at the interface between fundamental dairy research and the practical technological challenges facing the modern dairy industry worldwide. Topics addressed span the full range of dairy technologies, the production of diverse dairy products across the world and the development of dairy ingredients for food applications.