The role of chronic infection with toxoplasmosis in mitochondrial DNA 4,977 bp common deletion in sperm of men infertility.

Toxoplasma has the ability to infect a wide range of living organisms, including humans, and the infection found all over the world. The current study included 200 blood and semen samples collected from married men from which 100 samples of infertile men and 100 non-infertile men as control. The infertile men were divided into two groups: group A, which includes 35 (35%) infertile patients have IgG antibody sero-positive with toxoplasmosis, and group B, which includes 65 (65%) infertile people without toxoplasmosis were detected by rapid test for Toxo IgG/IgM antibody and then ensure by enzyme linked sorbent immunoassay (ELISA). The aging of men involved in the study extended between 20-57 years. The study revealed group A Toxoplasma IgG positive have mean concentration 65.10±13.84, was measured by ELISA in the serum of patients. Studies have shown mean age of men in group A and group B patients were 32.77±7.83 and 30.31±6.14, respectively. The age from 20-29 years as well as from 30 to 39 years high number of infertility in both A and B group patients. There is no significant distribution between group A and group B patients according to age of men infertility. The common deletion 4,977 bp mutation in mitochondrial sperm DNA(mt-DNA) was detected using the Gap-polymerase chain reaction (PCR) technique. Although the results showed that group A have high rate of common deletion mutation compare to group B but non-significant, while there is no deletion detection in the control group. The current results showed that reducing the mean total number of sperms in group A patient common deletion high significant compared to group B. The mean percentage of slow movement high significant than group B patient but non-significant mean percentage of death sperm although have high mean than to group B patient and abnormal sperm shape percentage have significant is higher in group A patient (common deletion) compared to group B.