COVID-19 与口面裂隙之间的关系。

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY General physiology and biophysics Pub Date : 2024-09-01 DOI:10.4149/gpb_2024024
Chao Song, Yan Zhang
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引用次数: 0

摘要

微RNA(miRNA)是基因表达的重要调节因子,与包括脊髓损伤(SCI)在内的各种病理过程有关。本研究旨在阐明 miR-10a 在 SCI 中的活性及其与 sirtuin 1(SIRT1)的潜在相互作用。建立了 SCI 大鼠模型,以评估后肢运动,测量 miR-10a、SIRT1、神经元存活和炎症因子的水平。此外,还建立了一个体外 SCI 细胞模型,以评估细胞存活率和炎症因子水平。研究验证了 miR10a 和 SIRT1 之间的相互作用。在 SCI 大鼠的组织中发现了 miR-10a 表达上调和 SIRT1 表达下调的现象。在 SCI 大鼠中敲除 miR-10a 能促进运动功能的恢复、提高神经元存活率并降低炎症细胞因子的水平。荧光素酶报告实验证实 miR-10a 直接靶向 SIRT1。在 PC12 细胞中,下调 miR-10a 可增加 SIRT1 的表达、提高细胞存活率并降低 LPS 刺激后的炎症因子水平。相反,敲除 SIRT1 会抑制下调的 miR-10a 对细胞活力和炎症反应的保护作用。研究结果表明,下调 miR-10a 可通过上调 SIRT1 的表达、改善功能恢复和减轻炎症反应来预防 SCI。靶向 miR-10a/SIRT1 轴是一种治疗 SCI 的有前途的策略。
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Down-regulated miR-10a protects against spinal cord injury by up-regulating SIRT1

MicroRNAs (miRNAs) are essential modulators of gene expression and are associated with various pathological processes, including spinal cord injury (SCI). This investigation aimed to elucidate miR-10a activity in SCI and its potential interaction with sirtuin 1 (SIRT1). The SCI rat model was established to assess hind limb movement, measure levels of miR-10a, SIRT1, neuronal survival, and inflammatory factors. An in-vitro SCI cell model was also developed to evaluate cell viability and inflammatory factor levels. The interaction between miR10a and SIRT1 was verified. Upregulated miR-10a and downregulated SIRT1 expression were found in the tissues of SCI rats. miR-10a knockdown in SCI rats enhanced the recovery of motor function, increased neuronal survival, and reduced the levels of inflammatory cytokines. Luciferase reporter assays confirmed that miR-10a targeted SIRT1 directly. In PC12 cells, downregulation of miR-10a increased SIRT1 expression, enhanced cell viability, and reduced inflammatory factor levels after LPS stimulation. Conversely, SIRT1 knockdown inhibited the protective effects of downregulated miR-10a on cell viability and inflammatory responses. The results suggest that miR-10a downregulation protects against SCI by upregulating SIRT1 expression, improving functional recovery, and reducing inflammation. Targeting the miR-10a/SIRT1 axis is a promising strategy for SCI treatment.

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来源期刊
General physiology and biophysics
General physiology and biophysics 生物-生化与分子生物学
CiteScore
2.70
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: General Physiology and Biophysics is devoted to the publication of original research papers concerned with general physiology, biophysics and biochemistry at the cellular and molecular level and is published quarterly by the Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences.
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