Shilia Jacob Kurian, Tejaswini Baral, Ruby Benson, Murali Munisamy, Kavitha Saravu, Gabriel Sunil Rodrigues, M. Sunil Krishna, Sahana Shetty, Amit Kumar, Sonal Sekhar Miraj
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引用次数: 0
摘要
本研究旨在发现南印度糖尿病足溃疡(DFU)患者体内维生素 D 受体(VDR)多态性(Fokl、Taql 和 Apal)与维生素 D 水平的关系。在这项病例对照研究中,70 例病例(DFU 患者)与来自南印度的 70 例糖尿病(DM [非DFU])患者和 70 例表面上健康的对照组(HC)的血浆维生素 D 水平和 VDR 基因型频率进行了比较。使用 ELISA 技术测量了血浆维生素 D 水平,并使用实时聚合酶链反应对 VDR 多态性进行了基因分型。逻辑回归用于发现 DFU 与 HC 和 DFU 与 DM 特征之间的关联。以年龄和性别为协变量,根据加性、显性和隐性模型进行了关联分析。结果显示,45.7%的DFU患者的维生素D水平足够,而DM患者和HC患者的这一比例分别为48.6%和40%。DFU与HC、DFU与DM的连锁不平衡分析表明,单核苷酸多态性(SNPs)Taq1(rs731236)和Apal(rs7975232)在DFU患者中存在强连锁不平衡。三组患者的等位基因和基因型频率相似。虽然加性模型没有显示出统计学意义,但年龄和性别与三个 SNP(Fokl、Taql 和 Apal)相关。在印度南部的 DFU 患者中,没有发现 VDR 基因多态性与维生素 D 水平之间存在关联。另一方面,年龄和性别与三个 SNPs 相关。
Association of vitamin D status and vitamin D receptor polymorphism in diabetic foot ulcer patients: A prospective observational study in a South-Indian tertiary healthcare facility
Objective of the study was to find the association of vitamin D receptor (VDR) polymorphisms (Fokl, Taql and Apal) with vitamin D levels in diabetic foot ulcer (DFU) patients in South India. In this case–control study, plasma vitamin D levels and VDR genotype frequencies of 70 cases (DFU patients) were compared with 70 diabetic (diabetes mellitus [DM] [non-DFU]) patients and 70 apparently healthy controls (HC) from South India. Plasma vitamin D levels were measured using the ELISA technique, and genotyping of VDR polymorphisms was carried out using real-time polymerase chain reaction. Logistic regression was used to find the association between DFU versus HC and DFU versus DM traits. Association analysis was performed based on additive, dominant and recessive models with age and gender as covariates. A 45.7% of DFU patients have sufficient vitamin D levels than 48.6% and 40% of DM patients and HC, respectively. Linkage disequilibrium analysis for DFU versus HC and DFU versus DM traits shows that single nucleotide polymorphisms (SNPs) Taq1 (rs731236) and Apal (rs7975232) are in strong linkage disequilibrium in DFU patients. The alleles and genotype frequencies were similar in all three groups. Although the additive model does not show statistical significance, age and sex correlate with the three SNPs (Fokl, Taql and Apal). No association was found between VDR gene polymorphisms and vitamin D levels in DFU patients in Southern India. On the other hand, age and sex correlate with the three SNPs.
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