抑制转谷氨酰胺酶 2 可抑制电离辐射诱导的体外皮肤角质细胞衰老。

IF 3.7 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY IUBMB Life Pub Date : 2024-08-14 DOI:10.1002/iub.2906
Juping Chen, Jiang Ma, Dandan Qi, Yuxuan Wang, Xiaoming Sun, Jinghui Yang, Wentao Sun, Changjiao Luan, Qing Shan, Weili Liu, Xingjie Ma
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引用次数: 0

摘要

衰老细胞的典型特征是分裂细胞的增殖稳定停止,并伴有衰老相关分泌表型(SASP)。皮肤细胞衰老是皮肤老化的主要原因,但由于缺乏已确定的皮肤衰老标志物,限制了我们对皮肤衰老相关机制的了解。最近的研究发现,细胞内钙信号已成为调节细胞衰老的关键因素。然而,钙信号在皮肤角质形成细胞衰老中的影响和作用仍只有部分了解。在这项研究中,我们利用电离辐射(I/R)刺激建立了一个皮肤角质细胞衰老模型,发现与正常对照组相比,钙相关基因转谷氨酰胺酶 2(TGM2)被显著诱导。有趣的是,通过抑制 I/R 促进的细胞内钙信号传导、活性氧(ROS)积累、DNA 损伤以及 NF-κB 介导的 SASP 分泌,发现抑制 TGM2 能延缓皮肤角质形成细胞的衰老。综上所述,我们的研究结果表明,抑制TGM2有助于绕过I/R诱导的皮肤角质细胞衰老,并揭示了应对I/R引起的皮肤压力的新策略。
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Inhibition of transglutaminase 2 inhibits ionizing radiation-induced cellular senescence in skin keratinocytes in vitro.

Senescent cells are typically characterized by a stable proliferation arrested in dividing cells accompanied with a senescence-associated secretory phenotype (SASP). Skin cellular senescence is the primary cause of skin aging, whereas the lack of identified skin senescence markers limits our understanding of the mechanisms involved in skin aging. Recent studies have revealed that intracellular calcium signaling has emerged as a key player in regulating cellular senescence and aging. However, the implication and roles of calcium signaling in skin keratinocyte senescence remain only partially understood. In this study, we developed a model for skin keratinocyte senescence using ionizing radiation (I/R) stimulation and found that the calcium-associated gene transglutaminase 2 (TGM2) was significantly induced compared with normal control. Interestingly, inhibition of TGM2 was found to delay skin keratinocyte senescence by suppressing I/R-promoted intracellular calcium signaling, accumulation of reactive oxygen species (ROS), DNA damage, as well as NF-κB-mediated SASP secretion. Taken together, our findings demonstrate that inhibition of TGM2 contributes to bypassing I/R-induced skin keratinocyte senescence and sheds light on novel strategies against skin stresses caused by I/R.

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来源期刊
IUBMB Life
IUBMB Life 生物-生化与分子生物学
CiteScore
10.60
自引率
0.00%
发文量
109
审稿时长
4-8 weeks
期刊介绍: IUBMB Life is the flagship journal of the International Union of Biochemistry and Molecular Biology and is devoted to the rapid publication of the most novel and significant original research articles, reviews, and hypotheses in the broadly defined fields of biochemistry, molecular biology, cell biology, and molecular medicine.
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