Carlos Arias-Mainer, Francisco Romero-Gavilán, Andreia Cerqueira, David Peñarocha-Oltra, Juan Carlos Bernabeu-Mira, Félix Elortza, Mikel Azkargorta, Mariló Gurruchaga, Isabel Goñi and Julio Suay
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Cytokine secretion, macrophage adhesion and gene expression were measured by ELISA, confocal microscopy and RT-PCR. Cell adhesion and collagen secretion were evaluated in HGF cultures. The adsorption of immune and regenerative proteins onto the surfaces was assessed employing <em>n</em>LC-MS/MS. MC04 + PA surfaces exhibited a change in the roughness, chemical composition and hydrophilicity of the material, showing more elongated HGF cells and a considerable increase in the area of cells exposed to the MC04 + PA surfaces. Moreover, cells cultured on MC04 + PA generally showed a reduction in the expression of proinflammatory genes (TNF-α, MCP-1, C5, NF-kB and ICAM-1) and an increase in the secretion of anti-inflammatory cytokines, such as IL-4. These results correlated with the proteomic data; we found preferential adsorption of proteins favouring cell adhesion, such as DSC1 and PCOC1. A considerable reduction in the adsorption of immunoglobulins and proteins associated with acute inflammatory response (including SAA4) was also observed. The study highlights the potential advantages of MC04 + PA surface treatment to modify dental implant abutments; it enhances their compatibility with soft tissues and reduces the inflammatory response.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Combining sandblasting and pink anodisation of Ti implants as a promising method for improving fibroblast adhesion and immune response†\",\"authors\":\"Carlos Arias-Mainer, Francisco Romero-Gavilán, Andreia Cerqueira, David Peñarocha-Oltra, Juan Carlos Bernabeu-Mira, Félix Elortza, Mikel Azkargorta, Mariló Gurruchaga, Isabel Goñi and Julio Suay\",\"doi\":\"10.1039/D4TB00992D\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >This study examined the effect of combining the sandblasting and anodising of titanium alloys used in implants on the cell response and protein adsorption patterns. 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引用次数: 0
摘要
本研究探讨了对用于植入物的钛合金进行喷砂和阳极氧化处理对细胞反应和蛋白质吸附模式的影响。根据表面处理的不同,钛合金样品被分为四组:机加工(MC)、粉红阳极氧化(PA)、喷砂(MC04)以及后两者的组合(MC04 + PA)。通过 SEM/EDX、拉曼、接触角测量和轮廓仪分析了它们的物理化学特性。使用人牙龈成纤维细胞(HGF)和 THP-1 巨噬细胞对体外反应进行了检测。通过 ELISA、共聚焦显微镜和 RT-PCR 测量了细胞因子分泌、巨噬细胞粘附和基因表达。在 HGF 培养物中对细胞粘附性和胶原分泌进行了评估。采用 nLC-MS/MS 评估了表面对免疫蛋白和再生蛋白的吸附情况。MC04 + PA 表面的粗糙度、化学成分和亲水性发生了变化,显示出更多拉长的 HGF 细胞,暴露在 MC04 + PA 表面的细胞面积显著增加。此外,在 MC04 + PA 上培养的细胞普遍显示促炎基因(TNF-α、MCP-1、C5、NF-kB 和 ICAM-1)的表达减少,而 IL-4 等抗炎细胞因子的分泌增加。这些结果与蛋白质组数据相关;我们发现了有利于细胞粘附的蛋白质的优先吸附,如 DSC1 和 PCOC1。我们还观察到,与急性炎症反应相关的免疫球蛋白和蛋白质(包括 SAA4)的吸附量大大减少。该研究强调了 MC04 + PA 表面处理在改良牙科种植基台方面的潜在优势;它增强了基台与软组织的兼容性,并降低了炎症反应。
Combining sandblasting and pink anodisation of Ti implants as a promising method for improving fibroblast adhesion and immune response†
This study examined the effect of combining the sandblasting and anodising of titanium alloys used in implants on the cell response and protein adsorption patterns. The titanium samples were divided into four groups depending on the surface treatment: machining (MC), pink anodisation (PA), sandblasting (MC04) and a combination of the last two (MC04 + PA). Their physicochemical properties were analysed by SEM/EDX, Raman, contact angle measurements and profilometry. In vitro responses were examined using human gingival fibroblastic (HGF) cells and THP-1 macrophages. Cytokine secretion, macrophage adhesion and gene expression were measured by ELISA, confocal microscopy and RT-PCR. Cell adhesion and collagen secretion were evaluated in HGF cultures. The adsorption of immune and regenerative proteins onto the surfaces was assessed employing nLC-MS/MS. MC04 + PA surfaces exhibited a change in the roughness, chemical composition and hydrophilicity of the material, showing more elongated HGF cells and a considerable increase in the area of cells exposed to the MC04 + PA surfaces. Moreover, cells cultured on MC04 + PA generally showed a reduction in the expression of proinflammatory genes (TNF-α, MCP-1, C5, NF-kB and ICAM-1) and an increase in the secretion of anti-inflammatory cytokines, such as IL-4. These results correlated with the proteomic data; we found preferential adsorption of proteins favouring cell adhesion, such as DSC1 and PCOC1. A considerable reduction in the adsorption of immunoglobulins and proteins associated with acute inflammatory response (including SAA4) was also observed. The study highlights the potential advantages of MC04 + PA surface treatment to modify dental implant abutments; it enhances their compatibility with soft tissues and reduces the inflammatory response.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices