以单细胞分辨率观察人体腹部皮下白色脂肪组织的老化。

IF 7.8 1区 医学 Q1 Biochemistry, Genetics and Molecular Biology Aging Cell Pub Date : 2024-08-14 DOI:10.1111/acel.14287
K. L. Whytock, A. Divoux, Y. Sun, M. F. Pino, G. Yu, C. A. Jin, J. J. Robino, A. Plekhanov, O. Varlamov, S. R. Smith, M. J. Walsh, L. M. Sparks
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引用次数: 0

摘要

白脂肪组织(WAT)是一个强大的能量储存和内分泌器官,随着年龄的增长对维持代谢健康至关重要。我们的目的是确定随着年龄增长白脂肪组织中出现的细胞特异性转录畸变。我们利用全长snRNA-Seq和组织学方法,在一个前瞻性队列中描述了人体腹部皮下WAT的细胞景观,该队列包括10名年轻个体(≤30岁)和10名老年个体(≥65岁),他们的性别和体重指数(BMI)保持平衡。与年轻组相比,老年组的胆固醇、极低密度脂蛋白、甘油三酯、促甲状腺激素和天冬氨酸转氨酶更高(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Aging human abdominal subcutaneous white adipose tissue at single cell resolution

White adipose tissue (WAT) is a robust energy storage and endocrine organ critical for maintaining metabolic health as we age. Our aim was to identify cell-specific transcriptional aberrations that occur in WAT with aging. We leveraged full-length snRNA-Seq and histology to characterize the cellular landscape of human abdominal subcutaneous WAT in a prospective cohort of 10 younger (≤30 years) and 10 older individuals (≥65 years) balanced for sex and body mass index (BMI). The older group had greater cholesterol, very-low-density lipoprotein, triglycerides, thyroid stimulating hormone, and aspartate transaminase compared to the younger group (p < 0.05). We highlight that aging WAT is associated with adipocyte hypertrophy, increased proportions of lipid-associated macrophages and mast cells, an upregulation of immune responses linked to fibrosis in pre-adipocyte, adipocyte, and vascular populations, and highlight CXCL14 as a biomarker of these processes. We show that older WAT has elevated levels of senescence marker p16 in adipocytes and identify the adipocyte subpopulation driving this senescence profile. We confirm that these transcriptional and phenotypical changes occur without overt fibrosis and in older individuals that have comparable WAT insulin sensitivity to the younger individuals.

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来源期刊
Aging Cell
Aging Cell 生物-老年医学
CiteScore
14.40
自引率
2.60%
发文量
212
审稿时长
8 weeks
期刊介绍: Aging Cell, an Open Access journal, delves into fundamental aspects of aging biology. It comprehensively explores geroscience, emphasizing research on the mechanisms underlying the aging process and the connections between aging and age-related diseases.
期刊最新文献
Issue Information Featured Cover Correction to ‘Increased transcriptome variation and localised DNA methylation changes in oocytes from aged mice revealed by parallel single-cell analysis’ RETRACTION: 1,25-Dihydroxyvitamin D exerts an antiaging role by activation of Nrf2-antioxidant signaling and inactivation of p16/p53-senescence signaling Issue Information
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