{"title":"寄生于印度曼尼普尔淡水蟹中的Microphallus sp.(Digenea: Microphallidae)的分子特征。","authors":"Voleentina Devi Athokpam, Lalit Mohan Goswami, Veena Tandon","doi":"10.1007/s12639-024-01694-y","DOIUrl":null,"url":null,"abstract":"<p><p>Freshwater crabs (<i>Potamiscus manipuriensis</i>), commonly consumed as local delicacies by the native people in the state of Manipur, were found to harbour metacercariae of <i>Microphallus</i> sp. (Family Microphyllidae), which were morphologically different from metacercariae of <i>Microphallus</i> spp reported earlier from different regions. So, PCR-based molecular characterization of this metacercaria was done utilizing rDNA marker regions: larger subunit (LSU) or 28S (D1-D3 region) and inter-transcribed spacer 2 (ITS2). Sequence and phylogenetic analyses confirmed that the taxon under study belonged to family Microphyllidae of genus <i>Microphallus</i>.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":"48 3","pages":"570-580"},"PeriodicalIF":0.0000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319564/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular characterization of <i>Microphallus</i> sp. (Digenea: Microphallidae) parasitic in freshwater crabs of Manipur, India.\",\"authors\":\"Voleentina Devi Athokpam, Lalit Mohan Goswami, Veena Tandon\",\"doi\":\"10.1007/s12639-024-01694-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Freshwater crabs (<i>Potamiscus manipuriensis</i>), commonly consumed as local delicacies by the native people in the state of Manipur, were found to harbour metacercariae of <i>Microphallus</i> sp. (Family Microphyllidae), which were morphologically different from metacercariae of <i>Microphallus</i> spp reported earlier from different regions. So, PCR-based molecular characterization of this metacercaria was done utilizing rDNA marker regions: larger subunit (LSU) or 28S (D1-D3 region) and inter-transcribed spacer 2 (ITS2). Sequence and phylogenetic analyses confirmed that the taxon under study belonged to family Microphyllidae of genus <i>Microphallus</i>.</p>\",\"PeriodicalId\":16664,\"journal\":{\"name\":\"Journal of Parasitic Diseases\",\"volume\":\"48 3\",\"pages\":\"570-580\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319564/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Parasitic Diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s12639-024-01694-y\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Immunology and Microbiology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Parasitic Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12639-024-01694-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/22 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
Molecular characterization of Microphallus sp. (Digenea: Microphallidae) parasitic in freshwater crabs of Manipur, India.
Freshwater crabs (Potamiscus manipuriensis), commonly consumed as local delicacies by the native people in the state of Manipur, were found to harbour metacercariae of Microphallus sp. (Family Microphyllidae), which were morphologically different from metacercariae of Microphallus spp reported earlier from different regions. So, PCR-based molecular characterization of this metacercaria was done utilizing rDNA marker regions: larger subunit (LSU) or 28S (D1-D3 region) and inter-transcribed spacer 2 (ITS2). Sequence and phylogenetic analyses confirmed that the taxon under study belonged to family Microphyllidae of genus Microphallus.
期刊介绍:
The primary constituency of the Journal of Parasitic Diseases is parasitology. It publishes original research papers (pure, applied and clinical), which contribute significantly to any area of parasitology. Research papers on various aspects of cellular and molecular parasitology are welcome.