Emily Andressa Santos Lima, Diefrey Ribeiro Campos, Eduardo Fellipe Melo Santos Soares, Anna Beatriz Ribeiro Fortunato, Taynara Monsores e Silva, Nayana de Figueiredo Pereira, Douglas Siqueira de Almeida Chaves, Yara Peluso Cid, Katherina Coumendouros
{"title":"Copaifera reticulata、Citrus paradisi、Lavandula hybrida 和 Salvia sclarea 的精油对栉水母幼虫和成虫的杀虫和驱虫活性。","authors":"Emily Andressa Santos Lima, Diefrey Ribeiro Campos, Eduardo Fellipe Melo Santos Soares, Anna Beatriz Ribeiro Fortunato, Taynara Monsores e Silva, Nayana de Figueiredo Pereira, Douglas Siqueira de Almeida Chaves, Yara Peluso Cid, Katherina Coumendouros","doi":"10.1007/s11686-024-00874-3","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>The flea <i>Ctenocephalides felis</i> (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from <i>Copaifera reticulata</i>, <i>Citrus paradisi</i>, <i>Lavandula hybrida</i> and <i>Salvia sclarea</i>, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy <i>in vitro</i> against <i>Ctenocephalides felis felis</i>.</p><h3>Methods</h3><p>Gas Chromatography with Flame Ionization Detector analyzed EO composition. <i>In vitro</i> tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10 cm<sup>2</sup> filter paper strip, each treated with 0.200 mL of the test solution. Immature stages activities were evaluated using 23.76 cm<sup>2</sup> discs of the same filter paper, each treated with 0.470 mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval.</p><h3>Results</h3><p>Major EO constituents were β-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of <i>C. paradisi</i>. All oils showed repellent activity at 800 μg/cm<sup>2</sup>. OECR exhibited greater residual efficacy.</p><h3>Conclusion</h3><p>Each EO demonstrated superior insecticidal activity against specific <i>C. felis felis</i> stages.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 3","pages":"1426 - 1438"},"PeriodicalIF":1.2000,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Insecticidal and Repellent Activity of Essential Oils from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea Against Immature and Adult Stages of Ctenocephalides felis felis\",\"authors\":\"Emily Andressa Santos Lima, Diefrey Ribeiro Campos, Eduardo Fellipe Melo Santos Soares, Anna Beatriz Ribeiro Fortunato, Taynara Monsores e Silva, Nayana de Figueiredo Pereira, Douglas Siqueira de Almeida Chaves, Yara Peluso Cid, Katherina Coumendouros\",\"doi\":\"10.1007/s11686-024-00874-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>The flea <i>Ctenocephalides felis</i> (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from <i>Copaifera reticulata</i>, <i>Citrus paradisi</i>, <i>Lavandula hybrida</i> and <i>Salvia sclarea</i>, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy <i>in vitro</i> against <i>Ctenocephalides felis felis</i>.</p><h3>Methods</h3><p>Gas Chromatography with Flame Ionization Detector analyzed EO composition. <i>In vitro</i> tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10 cm<sup>2</sup> filter paper strip, each treated with 0.200 mL of the test solution. Immature stages activities were evaluated using 23.76 cm<sup>2</sup> discs of the same filter paper, each treated with 0.470 mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval.</p><h3>Results</h3><p>Major EO constituents were β-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of <i>C. paradisi</i>. All oils showed repellent activity at 800 μg/cm<sup>2</sup>. OECR exhibited greater residual efficacy.</p><h3>Conclusion</h3><p>Each EO demonstrated superior insecticidal activity against specific <i>C. felis felis</i> stages.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"69 3\",\"pages\":\"1426 - 1438\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-024-00874-3\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-024-00874-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Insecticidal and Repellent Activity of Essential Oils from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea Against Immature and Adult Stages of Ctenocephalides felis felis
Purpose
The flea Ctenocephalides felis (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy in vitro against Ctenocephalides felis felis.
Methods
Gas Chromatography with Flame Ionization Detector analyzed EO composition. In vitro tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10 cm2 filter paper strip, each treated with 0.200 mL of the test solution. Immature stages activities were evaluated using 23.76 cm2 discs of the same filter paper, each treated with 0.470 mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval.
Results
Major EO constituents were β-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of C. paradisi. All oils showed repellent activity at 800 μg/cm2. OECR exhibited greater residual efficacy.
Conclusion
Each EO demonstrated superior insecticidal activity against specific C. felis felis stages.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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