研究炎症小体活性的传统方法和最新工具。

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of immunological methods Pub Date : 2024-08-13 DOI:10.1016/j.jim.2024.113744

Cassio Luiz Coutinho Almeida-da-Silva, Aline Cristina de Abreu Moreira-Souza, David M. Ojcius

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引用次数: 0

摘要

炎症小体在对感染的免疫反应、自身免疫性疾病的发展和癌症的控制中发挥着重要作用。本世纪初，人们开始使用 Western 印迹来描述炎症小体活化的特征。从那时起，人们开发了一系列技术来表征和观察细胞、组织和动物中的炎性体活化。这篇综述文章介绍了炎症小体领域研究人员最常用的技术，并提出组织或动物中炎症小体活化的细胞特异性表征可能很快就会被普遍报道。

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Traditional approaches and recent tools for studying inflammasome activity

Inflammasomes play a major role in the immune response to infection, development of autoimmune disease, and control of cancer. Western blots were originally used in the early 2000s to characterize inflammasome activation. Since then, a panoply of techniques has been developed to characterize and visualize inflammasome activation in cells, tissues, and animals. This review article describes the most common techniques used by researchers in the inflammasome field and proposes that cell-specific characterization of inflammasome activation in tissues or animals may soon be commonly reported.

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来源期刊

Journal of immunological methods 医学-免疫学

CiteScore

4.10

自引率

0.00%

发文量

120

审稿时长

3 months

期刊介绍： The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.