Silver-based bimetallic nanozyme fabrics with peroxidase-mimic activity for urinary glucose detection

The enhanced catalytic properties of bimetallic nanoparticles have been extensively investigated. In this study, bimetallic Ag-M (M = Au, Pt, or Pd) cotton fabrics were fabricated using a combination of electroless deposition and galvanic replacement reactions, and improvement in their peroxidase-mimicking catalytic activity compared to that of the parent Ag fabric was studied. The Ag-Pt bimetallic nanozyme fabric, which showed the highest catalytic activity and ability to simultaneously generate hydroxyl (•OH) and superoxide (O₂^•−) radicals, was assessed as a urine glucose sensor. This nanozyme fabric sensor could directly detect urinary glucose in the pathophysiologically relevant high millimolar range without requiring sample predilution. The sensor could achieve performance on par with that of the current clinical gold standard assay. These features of the Ag-Pt nanozyme sensor, particularly its ability to avoid interference effects from complex urinary matrices, position it as a viable candidate for point-of-care urinary glucose monitoring.

Graphical Abstract