雄性猕猴生殖组织中 SARS-CoV-2 进入分子 ACE2、NRP1、TMPRSS2 和 FURIN 的表达。

IF 2.1 4区 生物学 Q4 CELL BIOLOGY Histochemistry and Cell Biology Pub Date : 2024-12-01 Epub Date: 2024-08-17 DOI:10.1007/s00418-024-02314-9
Ryutaro Moriyama, Sho Nakamura, Ikki Mitsui, Makoto Sugiyama, Hirotaka Fukui, Hitomi Fukui, Teruki Hagiwara, Takako Miyabe-Nishiwaki, Juri Suzuki
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摘要

据报道,2019年冠状病毒病(COVID-19)通过导致精子发生功能障碍和抑制睾酮分泌来影响男性生殖功能。然而,COVID-19 与生殖功能受损之间的关系尚不清楚,例如这些对生殖功能的影响是严重急性呼吸系统综合征-冠状病毒-2(SARS-CoV-2)感染对男性生殖器官的直接影响,还是高烧的间接影响。在此,我们利用繁殖季节猕猴的睾丸和附属性腺研究了 SARS-CoV-2 的细胞进入分子(即 ACE2、NRP1、TMPRSS2 和 FURIN)是否在雄性生殖器官中表达。RT-PCR 表达分析表明,仅睾丸就表达了这四种分子。睾丸组织切片的免疫组化染色显示,ACE2表达于Leydig细胞和Sertoli细胞的顶端区域,而NRP1表达于Leydig细胞和Sertoli细胞核周围的细胞体。FURIN主要在Leydig细胞、次级精母细胞和精子中表达。但是,没有观察到 TMPRSS2 免疫阳性细胞。因此,无法在雄性灵长类动物的性腺和附属性腺中观察到表达所有四种分子的细胞。这些结果表明,SARS-CoV-2 不可能直接影响灵长类动物的精子发生,也不可能在曲细精管细胞中增殖,并通过以前已知的 ACE2 介导的感染途径释放到精液中。然而,在莱德细胞中观察到包括 ACE2 在内的三种分子的表达,这表明当灵长类动物(包括人类)感染 SARS-CoV-2 时,睾酮的合成和分泌可能会受到影响。
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Expression of SARS-CoV-2 entry molecules ACE2, NRP1, TMPRSS2, and FURIN in the reproductive tissues of male macaques.

Coronavirus disease 2019 (COVID-19) reportedly affects male reproductive function by causing spermatogenesis dysfunction and suppressing testosterone secretion. However, the relationship between COVID-19 and impaired reproductive function, such as whether these effects on reproductive function are a direct effect of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection in male reproductive organs or an indirect effect of high fever, is not known. Here, we examined whether the cell entry molecules of SARS-CoV-2, namely, ACE2, NRP1, TMPRSS2, and FURIN, are expressed in the male reproductive organs using the testes and accessory gonads of macaques during the breeding season. RT-PCR expression analysis showed that the testes alone expressed all four molecules. Immunohistochemical staining of testis tissue sections revealed that ACE2 is expressed in Leydig cells and the apical region of Sertoli cells, whereas NRP1 is expressed in the cell bodies surrounding the Leydig and Sertoli cell nuclei. FURIN is mainly expressed in Leydig cells, secondary spermatocytes, and spermatids. However, TMPRSS2 immunopositive cells were not observed. Therefore, it was not possible to observe cells expressing all four molecules in the gonads and accessory gonads of male primates. These results suggest that SARS-CoV-2 is unlikely to directly affect spermatogenesis in primates or proliferate in cells of the seminiferous tubules and undergo release into the semen through the previously known ACE2-mediated infection route. However, the expression of three molecules, including ACE2, was observed in Leydig cells, suggesting that testosterone synthesis and secretion may be affected when primates, including humans, are infected with SARS-CoV-2.

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来源期刊
Histochemistry and Cell Biology
Histochemistry and Cell Biology 生物-细胞生物学
CiteScore
4.90
自引率
8.70%
发文量
112
审稿时长
1 months
期刊介绍: Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.
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