Evaluation of cytotoxicity of silver nanoparticles on human skin melanoma SK- MEL-3 cell line: In vitro model

Globally the rate of melanoma skin cancer has increased in the recent years. Conventional treatments like chemotherapy, radiotherapy and surgery will damage the cancer cells and some healthy cells also in the body. These treatments are expensive and severe side effects on humans. Nanotechnology have recently been used in biological systems to enhance both reformative and translational therapies. In this study, we used binary solvent for preparation of plant extracts and phytosynthesized AgNPs. It is well known that AgNPs synthesized using green methods are preferred as eco-friendly, low cost, rapid and efficient process. For the assessment of the therapeutic activity of synthesized AgNPs, we proposed an in-vitro model of human melanoma skin cancerous cells (SK-MEL-3). The specific characteristics of AgNPs such as size, shape, distribution, crystalline structure etc. are examined before the medicinal application. In addition, we have done cytotoxicity analysis and apoptotic study to understand the morphological alteration in malignant cells.