脂质转运 ER 蛋白 TMEM24 和 C2CD2 与带 4.1 在细胞-细胞接触处的复合物。

IF 7.4 1区 生物学 Q1 CELL BIOLOGY Journal of Cell Biology Pub Date : 2024-11-04 Epub Date: 2024-08-19 DOI:10.1083/jcb.202311137
Ben Johnson, Maria Iuliano, TuKiet T Lam, Thomas Biederer, Pietro V De Camilli
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引用次数: 0

摘要

ER和质膜(PM)之间的连接与钙平衡、非囊泡脂质转移和其他细胞功能有关。脑富集的 TMEM24(C2CD2L)及其同源物 C2CD2 是两种既能通过多基态 C 端基团与质膜连接又能作为磷脂转运体的 ER 蛋白。我们报告说,这两种蛋白还与带状 4.1 家族成员形成复合物,而带状 4.1 家族成员又与包括细胞粘附分子(如 SynCAM 1)在内的 PM 蛋白结合。这种复合物在细胞接触部位富集了含有 ER/PM 连接的 TMEM24 和 C2CD2。当带 4.1 成为连接点的一部分时,依赖 TMEM24 的 ER/PM 连接点的动态特性会受到影响,因为细胞相邻 ER/PM 连接点上的 TMEM24 不会因钙升高而从 PM 上脱落,这与非细胞相邻连接点上的 TMEM24 不同。在细胞(包括神经元)与其他细胞接触的部位,TMEM24 和 C2CD2 在 ER 和 PM 之间的脂质运输可能参与了对细胞接触依赖性信号的适应性反应。
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A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell-cell contacts.

Junctions between the ER and plasma membrane (PM) are implicated in calcium homeostasis, non-vesicular lipid transfer, and other cellular functions. Two ER proteins that function both as tethers to the PM via a polybasic C-terminus motif and as phospholipid transporters are brain-enriched TMEM24 (C2CD2L) and its paralog C2CD2. We report that both proteins also form a complex with band 4.1 family members, which in turn bind PM proteins including cell adhesion molecules such as SynCAM 1. This complex enriches TMEM24 and C2CD2 containing ER/PM junctions at sites of cell contacts. Dynamic properties of TMEM24-dependent ER/PM junctions are impacted when band 4.1 is part of the junction, as TMEM24 at cell-adjacent ER/PM junctions is not shed from the PM by calcium rise, unlike TMEM24 at non-cell adjacent junctions. Lipid transport between the ER and the PM by TMEM24 and C2CD2 at sites where cells, including neurons, contact other cells may participate in adaptive responses to cell contact-dependent signaling.

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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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