ICP5249通过激活AMPK-自噬信号通路促进毛发生长

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of microbiology and biotechnology Pub Date : 2024-09-28 Epub Date: 2024-07-26 DOI:10.4014/jmb.2406.06015
Jung Ok Lee, Yu-Jin Kim, You Na Jang, Jung Min Lee, Kayoung Shin, Sekyoo Jeong, Hwa-Jee Chung, Beom Joon Kim
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引用次数: 0

摘要

自噬是调节毛发生长的关键。因此,我们开发了自噬激活剂 ICP5249(四羧甲基棕榈酰二肽五钠),并研究了它在头发生长中的潜在作用。我们使用体外人真皮乳头细胞(hDPCs)培养模型、人毛囊(hHFs)器官培养模型和毛发生长期小鼠模型评估了它对毛发生长的影响。ICP5249 增加了 hDPCs 的增殖和碱性磷酸酶(ALP)的表达。它还能提高微管相关蛋白(MAP)轻链3-II(LC3-II)的表达,增加AMP激活蛋白激酶α(AMPKα)和unc-51样激酶1(ULK1)在hDPCs中的磷酸化。ICP5249延长了hHFs的长度并增加了LC3 II的表达。同样,ICP5249 还能显著增加毛发生长期小鼠的毛发生长面积、真皮厚度以及生长期和休止期比率。此外,它还能上调小鼠背侧皮肤的 Ki-67 和 LC3-II 表达以及 AMPKα 磷酸化。为了研究 AMPK 是否调节 ICP5249 诱导的毛发生长,在使用化合物 C(AMPK 抑制剂)处理后,对 ICP5249 的活性进行了评估。结果表明,化合物 C 抑制了 ICP5249 介导的 hDPCs 增殖和毛发诱导。此外,化合物 C 还抑制了 ICP5249 介导的小鼠毛发生长面积、真皮厚度、生长期和休止期比例以及 LC3-II 的表达,这表明 ICP5249 通过调节自噬促进毛发生长,而 AMPKα 在这一过程中起着调节作用。综上所述,我们证明了 ICP5249 有潜力成为改善毛发生长的成分。
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ICP5249 Promotes Hair Growth by Activating the AMPK-Autophagy Signaling Pathway.
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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