{"title":"揭开无氧呼吸的神秘面纱:一个解决问题的练习。","authors":"Tomas Linder","doi":"10.1128/jmbe.00044-24","DOIUrl":null,"url":null,"abstract":"<p><p>Anaerobic respiration reactions are of fundamental importance to global biogeochemical cycling of elements. Yet, the idea that cellular respiration can occur not only in the absence of oxygen but also involve the oxidation of inorganic substrates (e.g., AsO<sub>3</sub><sup>3-</sup>, Fe<sup>2+</sup>, H<sub>2</sub>, H<sub>2</sub>S, Mn<sup>2+</sup>, NH<sub>3</sub>, and S<sup>0</sup>) is often foreign to many undergraduate students. This article describes a problem-solving exercise where students are introduced to the thermodynamic fundamentals of respiration with a particular focus on the role of redox (reduction-oxidation) potentials (<i>E<sub>0</sub>´</i>). In the exercise, the students investigate how the difference in redox potential (<i>ΔE<sub>0</sub>´</i>) between different pairs of reductants and oxidants affects the range of permissible microbial metabolic reactions in natural environments when oxygen is absent.</p>","PeriodicalId":46416,"journal":{"name":"Journal of Microbiology & Biology Education","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Demystifying anaerobic respiration: a problem-solving exercise.\",\"authors\":\"Tomas Linder\",\"doi\":\"10.1128/jmbe.00044-24\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Anaerobic respiration reactions are of fundamental importance to global biogeochemical cycling of elements. Yet, the idea that cellular respiration can occur not only in the absence of oxygen but also involve the oxidation of inorganic substrates (e.g., AsO<sub>3</sub><sup>3-</sup>, Fe<sup>2+</sup>, H<sub>2</sub>, H<sub>2</sub>S, Mn<sup>2+</sup>, NH<sub>3</sub>, and S<sup>0</sup>) is often foreign to many undergraduate students. This article describes a problem-solving exercise where students are introduced to the thermodynamic fundamentals of respiration with a particular focus on the role of redox (reduction-oxidation) potentials (<i>E<sub>0</sub>´</i>). In the exercise, the students investigate how the difference in redox potential (<i>ΔE<sub>0</sub>´</i>) between different pairs of reductants and oxidants affects the range of permissible microbial metabolic reactions in natural environments when oxygen is absent.</p>\",\"PeriodicalId\":46416,\"journal\":{\"name\":\"Journal of Microbiology & Biology Education\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-08-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Microbiology & Biology Education\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1128/jmbe.00044-24\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"EDUCATION, SCIENTIFIC DISCIPLINES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Microbiology & Biology Education","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1128/jmbe.00044-24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"EDUCATION, SCIENTIFIC DISCIPLINES","Score":null,"Total":0}
Demystifying anaerobic respiration: a problem-solving exercise.
Anaerobic respiration reactions are of fundamental importance to global biogeochemical cycling of elements. Yet, the idea that cellular respiration can occur not only in the absence of oxygen but also involve the oxidation of inorganic substrates (e.g., AsO33-, Fe2+, H2, H2S, Mn2+, NH3, and S0) is often foreign to many undergraduate students. This article describes a problem-solving exercise where students are introduced to the thermodynamic fundamentals of respiration with a particular focus on the role of redox (reduction-oxidation) potentials (E0´). In the exercise, the students investigate how the difference in redox potential (ΔE0´) between different pairs of reductants and oxidants affects the range of permissible microbial metabolic reactions in natural environments when oxygen is absent.