Li Wei*, Qian Lu, Hongshen Li, Dong Wei, Xinxin Zhang, Jia Ouyang, Chunying Li and Shizhong Li*,
{"title":"焦亚硫酸钠对盐碱地甜高粱青贮质量的影响:微生物多样性和元基因组分析","authors":"Li Wei*, Qian Lu, Hongshen Li, Dong Wei, Xinxin Zhang, Jia Ouyang, Chunying Li and Shizhong Li*, ","doi":"10.1021/acsagscitech.4c0010410.1021/acsagscitech.4c00104","DOIUrl":null,"url":null,"abstract":"<p >This study examined the impact of sodium pyrosulfite (Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub>) as a silage additive on the fermentation, quality, microbial diversity, and metabolic function of sweet sorghum silage in saline–alkali soil. Sweet sorghum grown in local saline–alkali soil was harvested, defoliated, and sprayed with a Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub> solution (contained 400–2000 ppm SO<sub>2</sub>), vacuum-sealed, and fermented at 25 °C. Samples were stored for 0–64 days and analyzed for chemical composition and microbial diversity. After 8 days, the pH of all silages dropped below 3.8. The 16th day silage chemical analysis revealed Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub> inhibiting ethanol and acetic acid production; S12 showed the best results: 5.497% total sugar, 2.357% lactic acid, 0.825% acetic acid, and 0.669% ethanol and achieved the highest silage quality scores (DB50/T 669-2016: 82, “Excellent”; DGL: 19, “Very Good”). Microbial analysis showed that Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub> inhibited spoilage microbes, reduced sugar consumption by nonlactic acid bacteria (such as genus <i>Rehenlla1</i>), and promoted the fermentation of lactic acid bacteria, and the original pathogenic genus did not hinder lactic acid fermentation. Predominant genera like <i>Rehenlla1</i> and <i>Lactobacillus</i> contributed the most to the key metabolic pathways of silage in the best treatment period.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":"4 8","pages":"791–805 791–805"},"PeriodicalIF":2.3000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Sodium Pyrosulfite on the Quality of Sweet Sorghum Silage in Saline–Alkali Soil: Microbial Diversity and Metagenomic Analysis\",\"authors\":\"Li Wei*, Qian Lu, Hongshen Li, Dong Wei, Xinxin Zhang, Jia Ouyang, Chunying Li and Shizhong Li*, \",\"doi\":\"10.1021/acsagscitech.4c0010410.1021/acsagscitech.4c00104\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >This study examined the impact of sodium pyrosulfite (Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub>) as a silage additive on the fermentation, quality, microbial diversity, and metabolic function of sweet sorghum silage in saline–alkali soil. Sweet sorghum grown in local saline–alkali soil was harvested, defoliated, and sprayed with a Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub> solution (contained 400–2000 ppm SO<sub>2</sub>), vacuum-sealed, and fermented at 25 °C. Samples were stored for 0–64 days and analyzed for chemical composition and microbial diversity. After 8 days, the pH of all silages dropped below 3.8. The 16th day silage chemical analysis revealed Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub> inhibiting ethanol and acetic acid production; S12 showed the best results: 5.497% total sugar, 2.357% lactic acid, 0.825% acetic acid, and 0.669% ethanol and achieved the highest silage quality scores (DB50/T 669-2016: 82, “Excellent”; DGL: 19, “Very Good”). Microbial analysis showed that Na<sub>2</sub>S<sub>2</sub>O<sub>5</sub> inhibited spoilage microbes, reduced sugar consumption by nonlactic acid bacteria (such as genus <i>Rehenlla1</i>), and promoted the fermentation of lactic acid bacteria, and the original pathogenic genus did not hinder lactic acid fermentation. Predominant genera like <i>Rehenlla1</i> and <i>Lactobacillus</i> contributed the most to the key metabolic pathways of silage in the best treatment period.</p>\",\"PeriodicalId\":93846,\"journal\":{\"name\":\"ACS agricultural science & technology\",\"volume\":\"4 8\",\"pages\":\"791–805 791–805\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS agricultural science & technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsagscitech.4c00104\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS agricultural science & technology","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsagscitech.4c00104","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Effect of Sodium Pyrosulfite on the Quality of Sweet Sorghum Silage in Saline–Alkali Soil: Microbial Diversity and Metagenomic Analysis
This study examined the impact of sodium pyrosulfite (Na2S2O5) as a silage additive on the fermentation, quality, microbial diversity, and metabolic function of sweet sorghum silage in saline–alkali soil. Sweet sorghum grown in local saline–alkali soil was harvested, defoliated, and sprayed with a Na2S2O5 solution (contained 400–2000 ppm SO2), vacuum-sealed, and fermented at 25 °C. Samples were stored for 0–64 days and analyzed for chemical composition and microbial diversity. After 8 days, the pH of all silages dropped below 3.8. The 16th day silage chemical analysis revealed Na2S2O5 inhibiting ethanol and acetic acid production; S12 showed the best results: 5.497% total sugar, 2.357% lactic acid, 0.825% acetic acid, and 0.669% ethanol and achieved the highest silage quality scores (DB50/T 669-2016: 82, “Excellent”; DGL: 19, “Very Good”). Microbial analysis showed that Na2S2O5 inhibited spoilage microbes, reduced sugar consumption by nonlactic acid bacteria (such as genus Rehenlla1), and promoted the fermentation of lactic acid bacteria, and the original pathogenic genus did not hinder lactic acid fermentation. Predominant genera like Rehenlla1 and Lactobacillus contributed the most to the key metabolic pathways of silage in the best treatment period.