焦亚硫酸钠对盐碱地甜高粱青贮质量的影响:微生物多样性和元基因组分析

IF 2.3 Q1 AGRICULTURE, MULTIDISCIPLINARY ACS agricultural science & technology Pub Date : 2024-07-26 DOI:10.1021/acsagscitech.4c0010410.1021/acsagscitech.4c00104
Li Wei*, Qian Lu, Hongshen Li, Dong Wei, Xinxin Zhang, Jia Ouyang, Chunying Li and Shizhong Li*, 
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引用次数: 0

摘要

本研究考察了焦亚硫酸钠(Na2S2O5)作为青贮添加剂对盐碱土壤中甜高粱青贮发酵、质量、微生物多样性和代谢功能的影响。将生长在当地盐碱土壤中的甜高粱收割、落叶、喷洒 Na2S2O5 溶液(含 400-2000 ppm SO2)、真空密封并在 25 °C 下发酵。样品储存 0-64 天,分析其化学成分和微生物多样性。8 天后,所有青贮饲料的 pH 值均降至 3.8 以下。第 16 天的青贮化学分析显示,Na2S2O5 抑制了乙醇和乙酸的产生;S12 的结果最好:S12 的结果最好:总糖 5.497%、乳酸 2.357%、乙酸 0.825% 和乙醇 0.669%,青贮质量得分最高(DB50/T 669-2016:82,"优";DGL:19,"很好")。微生物分析表明,Na2S2O5 可抑制腐败微生物,减少非乳酸菌(如 Rehenlla1 属)的糖分消耗,促进乳酸菌的发酵,而原病原菌属并不妨碍乳酸发酵。在最佳处理期,Rehenlla1 和乳酸菌等主要菌属对青贮饲料的关键代谢途径贡献最大。
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Effect of Sodium Pyrosulfite on the Quality of Sweet Sorghum Silage in Saline–Alkali Soil: Microbial Diversity and Metagenomic Analysis

This study examined the impact of sodium pyrosulfite (Na2S2O5) as a silage additive on the fermentation, quality, microbial diversity, and metabolic function of sweet sorghum silage in saline–alkali soil. Sweet sorghum grown in local saline–alkali soil was harvested, defoliated, and sprayed with a Na2S2O5 solution (contained 400–2000 ppm SO2), vacuum-sealed, and fermented at 25 °C. Samples were stored for 0–64 days and analyzed for chemical composition and microbial diversity. After 8 days, the pH of all silages dropped below 3.8. The 16th day silage chemical analysis revealed Na2S2O5 inhibiting ethanol and acetic acid production; S12 showed the best results: 5.497% total sugar, 2.357% lactic acid, 0.825% acetic acid, and 0.669% ethanol and achieved the highest silage quality scores (DB50/T 669-2016: 82, “Excellent”; DGL: 19, “Very Good”). Microbial analysis showed that Na2S2O5 inhibited spoilage microbes, reduced sugar consumption by nonlactic acid bacteria (such as genus Rehenlla1), and promoted the fermentation of lactic acid bacteria, and the original pathogenic genus did not hinder lactic acid fermentation. Predominant genera like Rehenlla1 and Lactobacillus contributed the most to the key metabolic pathways of silage in the best treatment period.

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