创新益生菌制剂用于局部治疗糖尿病溃疡的潜在治疗策略:一项体内研究。

IF 4.6 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Nutrition & Diabetes Pub Date : 2024-08-20 DOI:10.1038/s41387-024-00320-3
Farkhonde Karimi, Nima Montazeri-Najafabady, Fatemeh Mohammadi, Amir Azadi, Farhad Koohpeyma, Ahmad Gholami
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引用次数: 0

摘要

背景:最近,乳酸菌的益生潜能已被用于研究从胃肠道疾病到抗生素耐药性感染等各种医疗并发症。此外,众所周知,糖尿病溃疡(DU)是全球最重要的医疗保健问题之一,全面影响着这些患者的生活质量。鉴于糖尿病溃疡的传统治疗方法无法完全预防后期并发症,开发替代疗法似乎至关重要:方法:我们设计了基于油凝胶的稳定益生菌细胞制剂,包括鼠李糖乳杆菌(L. rhamnosus)、干酪乳杆菌(L. casei)、发酵乳杆菌(L. fermentum)和嗜酸乳杆菌(L. acidophilus),作为一项体内研究来探讨它们对伤口愈合过程的影响。研究人员密切监测了两周内伤口修复过程中的形态、生化和组织病理学变化,并将其与局部使用四环素作为抗生素的效果进行了比较。此外,还评估了益生菌对一些常见病原体的抗生物膜活性:结果:研究结果表明,与抗生素组和对照组相比,油凝胶配方中的所有受试乳酸菌组(不包括干酪乳杆菌)都具有修复受损皮肤的巨大潜力,因为组织样本中的羟脯氨酸含量更高,成熟成纤维细胞的数量密度更高,毛囊、胶原纤维和新生血管的体积密度也更高。嗜酸乳杆菌和鼠李糖杆菌在所有乳酸杆菌种类、四环素治疗组和对照组中显示出最佳的伤口愈合潜力。此外,鼠李糖杆菌对测试病原体具有显著的生物膜抑制活性:本实验表明,所设计的含有益生菌(尤其是嗜酸乳杆菌和鼠李糖杆菌)的配方在控制糖尿病伤口愈合方面发挥了重要作用。尽管还需要在详细的临床试验中进行进一步的研究,但它可以作为糖尿病伤口愈合替代方法的一个令人鼓舞的提名。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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A potential therapeutic strategy of an innovative probiotic formulation toward topical treatment of diabetic ulcer: an in vivo study.

Background: The probiotic potential of Lacticacid bacteria has been studied in various medical complications, from gastrointestinal diseases to antibiotic resistance infections recently. Moreover, diabetic ulcer (DU) is known as one of the most significant global healthcare concerns, which comprehensively impacts the quality of life for these patients. Given that the conventional treatments of DUs have failed to prevent later complications completely, developing alternative therapies seems to be crucial.

Methods: We designed the stable oleogel-based formulation of viable probiotic cells, including Lactobacillus rhamnosus (L. rhamnosus), Lactobacillus casei (L. casei), Lactobacillus fermentum (L. fermentum), and Lactobacillus acidophilus (L. acidophilus) individually to investigate their effect on wound healing process as an in vivo study. The wound repair process was closely monitored regarding morphology, biochemical, and histopathological changes over two weeks and compared it with the effects of topical tetracycline as an antibiotic approach. Furthermore, the antibiofilm activity of probiotic bacteria was assessed against some common pathogens.

Results: The findings indicated that all tested lactobacillus groups (excluded L. casei) included in the oleogel-based formulation revealed a high potential for repairing damaged skin due to the considerably more levels of hydroxyproline content of tissue samples along with the higher numerical density of mature fibroblasts cell and volume density of hair follicles, collagen fibrils, and neovascularization in comparison with antibiotic and control groups. L. acidophilus and L. rhamnosus showed the best potential of wound healing among all lactobacillus species, groups treated by tetracycline and control groups. Besides, L. rhamnosus showed a significant biofilm inhibition activity against tested pathogens.

Conclusions: This experiment demonstrated that the designed formulations containing probiotics, particularly L. acidophilus and L. rhamnosus, play a central role in manipulating diabetic wound healing. It could be suggested as an encouraging nominee for diabetic wound-healing alternative approaches, though further studies in detailed clinical trials are needed.

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来源期刊
Nutrition & Diabetes
Nutrition & Diabetes ENDOCRINOLOGY & METABOLISM-NUTRITION & DIETETICS
CiteScore
9.20
自引率
0.00%
发文量
50
审稿时长
>12 weeks
期刊介绍: Nutrition & Diabetes is a peer-reviewed, online, open access journal bringing to the fore outstanding research in the areas of nutrition and chronic disease, including diabetes, from the molecular to the population level.
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