MiR-302c-3p 通过 LATS2/YAP 轴调节鸡卵巢颗粒细胞的自噬和凋亡

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY Theriogenology Pub Date : 2024-08-16 DOI:10.1016/j.theriogenology.2024.08.020
Peng Ren , Kejun Wu , Meiying Chen , Qinke Huang , Zhengwei Luo , Ye Wang
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引用次数: 0

摘要

母鸡卵泡闭锁的退化过程自然是从颗粒细胞开始的,这严重影响了蛋鸡的繁殖性能。过去的研究表明,颗粒细胞自噬和凋亡共同导致了卵泡闭锁。最新研究表明,miRNA 可调控颗粒细胞的自噬和凋亡,从而导致卵泡闭锁的发生。然而,miR-302c-3p在卵泡闭锁和发育中的作用仍不清楚。在这项采用 RNA-seq 方法的研究中,我们发现 miR-302c-3p 在萎缩卵泡中的表达明显下降,这表明它参与了卵泡闭锁的过程。随后,我们进行了体外研究,证实 miR-302c-3p 可抑制鸡颗粒细胞的自噬和凋亡。从机理上讲,LATS2 被认为是 miR-302c-3p 的假定靶基因,而且已有研究证明 LATS2 在调节鸡颗粒细胞的自噬和凋亡过程中发挥了积极的调控作用。此外,我们还验证了 miR-302c-3p 在鸡颗粒细胞中通过 LATS2-YAP 信号通路的调控功能。我们的研究结果共同证明,miR-302c-3p 以 LATS2 为靶点调节 YAP 信号通路,影响颗粒细胞的自噬和凋亡,导致卵泡闭锁。
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MiR-302c-3p regulates autophagy and apoptosis in ovarian granulosa cells via the LATS2/YAP axis in chickens

The degenerative process of follicular atresia in hens naturally commences in granulosa cells, significantly impacting laying hens' reproductive performance. Past studies suggested that granulosa cell autophagy and apoptosis work together to cause follicular atresia. Recent research indicates that miRNA regulates granulosa autophagy and apoptosis, which contributes to the development of follicular atresia. However, the role of miR-302c-3p in follicular atresia and development remains unclear. In this study with the RNA-seq approach, we found that miR-302c-3p expression was significantly decreased in atrophic follicles, suggesting its involvement in the follicular atresia process. Following this, we performed in vitro studies to confirm that miR-302c-3p inhibits autophagy and apoptosis in chicken granulosa cells. Mechanistically, LATS2 is considered as the putative target gene of miR-302c-3p, and it has been demonstrated that LATS2 exerts a positive regulatory role in the modulation of autophagy and apoptosis in chicken granulosa cells. Furthermore, we verified the regulatory function of miR-302c-3p in chicken granulosa cells via the LATS2-YAP signaling pathway. Our results collectively demonstrates that miR-302c-3p targets LATS2 to modulate the YAP signaling pathway, impacting autophagy and apoptosis in granulosa cells leading to follicular atresia.

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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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