Guilherme M Prado, Júlio César S Prado, Francisca Lidiane L DE Aguiar, Francisco Cesar B Barbosa, Jean P C DO Vale, Maria Rosário Martins, Silva Macedo Arantes, Natália V DE Sousa, Danielle M Lima, Emmanuel S Marinho, Márcia M Marinho, Raquel O S Fontenelle
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引用次数: 0
摘要
巴西以其广泛的植物生物多样性而闻名，重点是具有广泛抗菌潜力的 Cymbopogon、C. citratus 和 C.nardus。由白色念珠菌引起的念珠菌病在免疫抑制人群中非常普遍，并与医疗设备上的生物膜感染有关。本研究旨在评估柠檬香精油（C. citratus）和纳豆香精油（C. nardus）对浮游和生物膜形式的白色念珠菌的抗菌潜力。精油通过水蒸馏获得，化学成分通过 GC-FID 和 GC-MS 进行评估。采用肉汤微稀释法测定了最低抑制浓度，并通过棋盘试验评估了精油和两性霉素 B 的协同作用。生物膜活性通过 XTT 试验确定。用 VERO 细胞进行了细胞毒性试验，并进行了分子对接，以预测精油对 SAP-5 酶位点的相互作用效果。结果表明，精油对白僵菌的浮游细胞和生物膜具有活性。此外，精油还具有协同效应和低细胞毒性。分子对接显示，Cadinene、Caryophyllen oxide、Germacrene D 与 SAP-5 之间存在相互作用。研究结果表明，所研究的伞形科植物具有抗念珠菌的作用，有望进一步应用于治疗由白念珠菌引起的感染。
Antifungal, molecular docking and cytotoxic effect of the essential oil of Cymbopogon citratus (DC) Stapf. and Cymbopogon nardus (L.) Rendle against Candida albicans.
Brazil is renowned for its extensive plant biodiversity, with emphasis on Cymbopogon, C. citratus and C. nardus, with broad antimicrobial potential. Candidemias caused by Candida albicans are highly prevalent in immunosuppressed individuals and are associated with infections by biofilms on medical devices. The aim of this study was to evaluate the antimicrobial potential of essential oils C. citratus and C. nardus against C. albicans in planktonic and biofilm forms. Essential oils were obtained by hydrodistillation and chemical composition evaluated by GC-FID and GC-MS. The minimum inhibitory concentration was determined by the broth microdilution method and the synergy effect of essential oils and amphotericin B were evaluated by the checkerboard test. Biofilm activity was determined by the XTT assay. Cytotoxicity assays performed with VERO cells and molecular docking were performed to predict the effect of oil interaction on the SAP-5 enzyme site. The results showed activity of essential oils against planktonic cells and biofilm of C. albicans. Furthermore, the oils had a synergistic effect, and low cytotoxicity. Molecular docking showed interaction between Cadinene, Caryophyllen oxide, Germacrene D with SAP-5. The results indicate that Cymbopogon spp. studied are anti-Candida, with potential for further application in therapy against infections caused by C. albicans.
期刊介绍：
The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence.
Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.
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