小脑在蜜蜂(Apis mellifera)工蜂的成虫大脑发育过程中发挥作用。

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2024-08-21 DOI:10.1111/imb.12955
Juliana Ramos Martins, Izabella Cristina Silva, Talita Sarah Mazzoni, Gabriela Helena de Barrios, Flávia Cristina de Paula Freitas, Angel Roberto Barchuk
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引用次数: 0

摘要

成年蜜蜂(Apis mellifera)工蜂的大脑比蜂王的大,这有利于不同种姓之间的行为分化。这种大脑二重性是在幼虫-成虫阶段形成的,由种姓特异性基因表达级联驱动,以响应独特的激素环境。之前的分子筛选发现,小脑(mnb;DYRK1A)是这一过程中的潜在调控因子。在这里,我们使用 RNAi 方法降低了 mnb 的转录水平,并测试了其在蜜蜂脑双核发育过程中的作用。给白眼无色素角质层工蜂蛹注射mnb(Mnb-i)或gfp的dsRNA,并在2天和8天后使用经典的组织学和转录组分析评估它们的表型。注射 2 天后,Mnb-i 蜜蜂的 mnb 转录物下调了 98%。8 天后,Mnb-i 蜜蜂的大脑总体积以及蘑菇体(MB)、触角和视叶的体积都有所减少。此外,在蘑菇体的凯尼恩细胞区域观察到了凋亡迹象,脑组织的凝聚力也受到了影响。我们的转录组分析表明,有226个基因受到了mnb转录本敲除的影响,其中大部分基因允许轴突分束。这些结果表明,进化保守的mnb基因已被利用来促进激素介导的蜜蜂大脑形态可塑性发育,从而产生种性二重性。
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Minibrain plays a role in the adult brain development of honeybee (Apis mellifera) workers.

The brain of adult honeybee (Apis mellifera) workers is larger than that of queens, facilitating behavioural differentiation between the castes. This brain diphenism develops during the pharate-adult stage and is driven by a caste-specific gene expression cascade in response to unique hormonal milieus. Previous molecular screening identified minibrain (mnb; DYRK1A) as a potential regulator in this process. Here, we used RNAi approach to reduce mnb transcript levels and test its role on brain diphenism development in honeybees. White-eyed unpigmented cuticle worker pupae were injected with dsRNA for mnb (Mnb-i) or gfp, and their phenotypes were assessed two and 8 days later using classic histological and transcriptomic analyses. After 2 days of the injections, Mnb-i bees showed 98% of downregulation of mnb transcripts. After 8 days, the brain of Mnb-i bees showed reduction in total volume and in the volume of the mushroom bodies (MB), antennal, and optic lobes. Additionally, signs of apoptosis were observed in the Kenyon cells region of the MB, and the cohesion of the brain tissues was affected. Our transcriptomic analyses revealed that 226 genes were affected by the knockdown of mnb transcripts, most of which allowing axonal fasciculation. These results suggest the evolutionary conserved mnb gene has been co-opted for promoting hormone-mediated developmental brain morphological plasticity generating caste diphenism in honeybees.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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