利用 HPLC-MS/MS 同时分析家禽粪便中的抗菌剂残留和污染物:环境和食品安全监控工具。

IF 2.3 3区 农林科学 Q2 CHEMISTRY, APPLIED Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2024-11-01 Epub Date: 2024-08-23 DOI:10.1080/19440049.2024.2393334
Lina Trincado, Paula Cortés, Ekaterina Pokrant, María José Navarrete, Lisette Lapierre, Matías Maturana, Andrés Flores, Aldo Maddaleno, Javiera Cornejo
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引用次数: 0

摘要

动物粪便是一种潜在的污染危险,因为它可能藏有污染物,如抗菌剂残留、霉菌毒素和杀虫剂,从而对公众、动物和环境健康造成危害。为了评估这种风险,15 只实验肉鸡口服了污染物,以评估排泄水平。此前已开发出一种分析方法,利用高效液相色谱-串联质谱仪(HPLC-MS/MS)检测家禽粪便中的 18 种物质。污染物包括四环素、4-表-四环素、土霉素、4-表-氧四环素、金霉素、4-表-金霉素、泰乐菌素、红霉素、恩诺沙星、环丙沙星、氟甲喹、氟苯脲、用 EDTA-McIlvain 和乙腈提取氟甲胺喹、氟苯尼考、磺胺氯哒嗪、磺胺嘧啶、2,4-二氯苯氧乙酸、玉米赤霉烯酮、α-和β-玉米赤霉烯醇。该方法对所有分析物的定标曲线线性关系均良好,P值小于0.05,RSD小于25%,R2大于0.95。根据欧盟文件2021/808/EC、技术报告CEN/TR 16059、SANTE/11813/2017以及国际兽医协调会议(VICH GL2和GL2)验证了该方法的定量限、选择性、确证判定限、基质效应、精密度和回收率参数:VICH GL2 和 GL49。该方法可在给药后 12-36 h 检测到大多数分析物,并同时检测和量化混合污染物。因此,家禽粪便是动物生产中污染物在屠宰前传播的潜在基质,对其进行控制将最大限度地减少对环境的影响并减轻抗菌药耐药性。
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Simultaneous analysis of antimicrobial residues and contaminants in poultry droppings by HPLC-MS/MS: a tool for environmental and food safety monitoring.

Animal waste is a potential pollution hazard as it can harbour contaminants, such as antimicrobial residues, mycotoxins, and pesticides, becoming a risk to the public, animal, and environmental health. To assess this risk, 15 experimental broiler chickens orally received contaminants to evaluate excretion levels. An analytical method was previously developed to detect 18 substances in poultry droppings using high-performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS). Contaminants including tetracycline, 4-epi-tetracycline, oxytetracycline, 4-epi-oxytetracycline, chlortetracycline, 4-epi-chlortetracycline, tylosin, erythromycin, enrofloxacin, ciprofloxacin, flumequine, florfenicol, sulfachloropyridazine, sulfadiazine, 2,4-dichlorophenoxyacetic acid, zearalenone, alpha- and beta-zearalenol, were extracted with EDTA-McIlvain and acetonitrile. This method showed a p-value < 0.05, RSD < 25%, and R2 > 0.95 in the calibration curves linearity for all analytes. The limit of quantification, selectivity, decision limit for confirmation, matrix effect, precision, and recovery parameters were validated according to European Union document 2021/808/EC, technical report CEN/TR 16059, SANTE/11813/2017 and according to the Veterinary International Conference on Harmonization: VICH GL2 and GL49. This method confirmed the detection of most analytes 12-36 h post-administration and simultaneously detected and quantified mixed contaminants. Thereby, poultry droppings are a potential matrix for spreading contaminants in animal production before slaughter and their control will minimize environmental impacts and mitigate antimicrobial resistance.

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来源期刊
CiteScore
7.40
自引率
6.90%
发文量
136
审稿时长
3 months
期刊介绍: Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.
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