Jin Seok Kim, Hyo-Won Jeong, Young Hee Jin, JinWoo Kim, Jin-ah Kim, Sook Hyun Park, So-Mi Yoon, Sang-Eun Jung, Jung Im Jang, Eun Ji Kim, Jae In Lee, Jib-Ho Lee
{"title":"从首尔废水中分离出的一种 adecarboxylata Leclercia 菌株中出现了可移动的可乐定抗性基因 mcr-1。","authors":"Jin Seok Kim, Hyo-Won Jeong, Young Hee Jin, JinWoo Kim, Jin-ah Kim, Sook Hyun Park, So-Mi Yoon, Sang-Eun Jung, Jung Im Jang, Eun Ji Kim, Jae In Lee, Jib-Ho Lee","doi":"10.1016/j.jgar.2024.08.003","DOIUrl":null,"url":null,"abstract":"<div><p>Colistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene <em>mcr-1</em> has become a public health threat. In this study, we identified <em>mcr-1</em>-harboring <em>Leclercia adecarboxylata</em> strain (WWCOL-134) isolated from wastewater in Seoul. The strain had a colistin MIC value of 2 µg/ml and was resistant to cefotaxime, gentamicin, tetracycline, trimethoprim and sulfamethoxazole. The <em>mcr-1</em> gene, along with an array of resistance genes, was located on a 236-kb plasmid (pCOL134-1), which contained the typical IncHI2 backbone of reported <em>mcr-1</em>-carrying plasmids, and was transferred to an <em>Escherichia coli</em> strain by conjugation. To the best of our knowledge, this is the first study to report the emergence of <em>mcr-1</em>-harboring <em>Leclercia</em> sp. isolate. Our findings demonstrate the ongoing spread of colistin resistance among Enterobacterales species, emphasizing the need for surveillance of antimicrobial resistance in wastewater environments.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 37-40"},"PeriodicalIF":3.7000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001565/pdfft?md5=98329aa4dee120a1d560bf25a4abc935&pid=1-s2.0-S2213716524001565-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul\",\"authors\":\"Jin Seok Kim, Hyo-Won Jeong, Young Hee Jin, JinWoo Kim, Jin-ah Kim, Sook Hyun Park, So-Mi Yoon, Sang-Eun Jung, Jung Im Jang, Eun Ji Kim, Jae In Lee, Jib-Ho Lee\",\"doi\":\"10.1016/j.jgar.2024.08.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Colistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene <em>mcr-1</em> has become a public health threat. In this study, we identified <em>mcr-1</em>-harboring <em>Leclercia adecarboxylata</em> strain (WWCOL-134) isolated from wastewater in Seoul. The strain had a colistin MIC value of 2 µg/ml and was resistant to cefotaxime, gentamicin, tetracycline, trimethoprim and sulfamethoxazole. The <em>mcr-1</em> gene, along with an array of resistance genes, was located on a 236-kb plasmid (pCOL134-1), which contained the typical IncHI2 backbone of reported <em>mcr-1</em>-carrying plasmids, and was transferred to an <em>Escherichia coli</em> strain by conjugation. To the best of our knowledge, this is the first study to report the emergence of <em>mcr-1</em>-harboring <em>Leclercia</em> sp. isolate. Our findings demonstrate the ongoing spread of colistin resistance among Enterobacterales species, emphasizing the need for surveillance of antimicrobial resistance in wastewater environments.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"39 \",\"pages\":\"Pages 37-40\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-08-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001565/pdfft?md5=98329aa4dee120a1d560bf25a4abc935&pid=1-s2.0-S2213716524001565-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001565\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001565","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul
Colistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene mcr-1 has become a public health threat. In this study, we identified mcr-1-harboring Leclercia adecarboxylata strain (WWCOL-134) isolated from wastewater in Seoul. The strain had a colistin MIC value of 2 µg/ml and was resistant to cefotaxime, gentamicin, tetracycline, trimethoprim and sulfamethoxazole. The mcr-1 gene, along with an array of resistance genes, was located on a 236-kb plasmid (pCOL134-1), which contained the typical IncHI2 backbone of reported mcr-1-carrying plasmids, and was transferred to an Escherichia coli strain by conjugation. To the best of our knowledge, this is the first study to report the emergence of mcr-1-harboring Leclercia sp. isolate. Our findings demonstrate the ongoing spread of colistin resistance among Enterobacterales species, emphasizing the need for surveillance of antimicrobial resistance in wastewater environments.
期刊介绍:
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.