Fatma Koc, Claire Magner, Kiera Murphy, Sean T Kelleher, Mong H Tan, Molly O'Toole, Dominic Jenkins, Jordan Boyle, Marie Lavelle, Niamh Maguire, Paul R Ross, Catherine Stanton, Colin J McMahon
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Faecal samples were collected from infants alongside the collection of demographic and clinical data in order to examine gut microbiome changes before and after surgery. 16S rRNA sequencing analysis was performed on DNA isolated from stool samples to determine changes in gut microbiome composition. Thirty-three patients were recruited, with samples from thirteen of these available for final analysis. Compared with healthy, matched controls, at a genus level, pre-operative samples for infants with CHD demonstrated a higher relative abundance of Escherichia-Shigella (31% vs 2-6%) and a lower relative abundance of Bifidobacterium (13% vs 40-60%). In post-operative samples, the relative abundance of Escherichia-Shigella (35%), Enterococcus (11%), Akkermansia (6%), and Staphylococcus (5%) were higher than pre-op samples. One infant developed post-operative necrotising-enterocolitis (NEC). They displayed a marked abundance of the Enterococcus (93%) genus pre-operatively. 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引用次数: 0
摘要
患有先天性心脏病(CHD)的婴儿在接受心肺搭桥手术(CPB)时,其肠道微生物组可能会发生严重改变。本研究旨在检查搭桥手术前后的肠道微生物组,以探讨肠道生物多样性改变的潜在影响。这项前瞻性队列研究涉及接受 CPB 的患有先天性心脏病的婴儿。在收集人口统计学和临床数据的同时,还收集了婴儿的粪便样本,以研究手术前后肠道微生物组的变化。对从粪便样本中分离出的 DNA 进行了 16S rRNA 测序分析,以确定肠道微生物组组成的变化。共招募了 33 名患者,其中 13 名患者的样本可用于最终分析。与健康的匹配对照组相比,在属种水平上,患有先天性心脏病的婴儿的术前样本中,埃希氏菌的相对丰度较高(31% 对 2-6%),而双歧杆菌的相对丰度较低(13% 对 40-60%)。在术后样本中,埃希氏菌(35%)、肠球菌(11%)、Akkermansia(6%)和葡萄球菌(5%)的相对含量高于术前样本。一名婴儿术后出现坏死性小肠结肠炎(NEC)。术前,他们体内的肠球菌属明显增多(93%)。这项研究表明,与健康对照组相比,患有先天性心脏病的婴儿的肠道微生物群发生了改变,大量毒性菌种与 NEC 之间可能存在联系。
Gut Microbiome in Children with Congenital Heart Disease After Cardiopulmonary Bypass Surgery (GuMiBear Study).
The gut microbiome of infants with congenital heart disease (CHD) undergoing cardiopulmonary bypass surgery (CPB) is at risk of profound alteration. The aim of this study was to examine the gut microbiome pre- and post-bypass surgery to explore potential implications of altered gut biodiversity. A prospective cohort study involving infants with CHD who underwent CPB was performed. Faecal samples were collected from infants alongside the collection of demographic and clinical data in order to examine gut microbiome changes before and after surgery. 16S rRNA sequencing analysis was performed on DNA isolated from stool samples to determine changes in gut microbiome composition. Thirty-three patients were recruited, with samples from thirteen of these available for final analysis. Compared with healthy, matched controls, at a genus level, pre-operative samples for infants with CHD demonstrated a higher relative abundance of Escherichia-Shigella (31% vs 2-6%) and a lower relative abundance of Bifidobacterium (13% vs 40-60%). In post-operative samples, the relative abundance of Escherichia-Shigella (35%), Enterococcus (11%), Akkermansia (6%), and Staphylococcus (5%) were higher than pre-op samples. One infant developed post-operative necrotising-enterocolitis (NEC). They displayed a marked abundance of the Enterococcus (93%) genus pre-operatively. This study demonstrates that infants with CHD have an altered gut microbiome when compared with healthy controls and there might be a possible link between an abundance of virulent species and NEC.
期刊介绍:
The editor of Pediatric Cardiology welcomes original manuscripts concerning all aspects of heart disease in infants, children, and adolescents, including embryology and anatomy, physiology and pharmacology, biochemistry, pathology, genetics, radiology, clinical aspects, investigative cardiology, electrophysiology and echocardiography, and cardiac surgery. Articles which may include original articles, review articles, letters to the editor etc., must be written in English and must be submitted solely to Pediatric Cardiology.