利用质谱法快速鉴定 SARS CoV-2 omicron 亚变体 JN.1 (BA.2.86.1.1)

IF 3.1 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY Journal of Mass Spectrometry and Advances in the Clinical Lab Pub Date : 2024-08-01 DOI:10.1016/j.jmsacl.2024.08.003

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引用次数: 0

摘要

目的利用选择离子监测（SIM）和高分辨质谱法证明了对 BA.2.86 系菌株（包括新的亚变异株 JN.1（BA.2.86.1.1））的快速检测和区分。结果与 L455S 突变相关的表面尖峰蛋白中的肽特异性标记在蛋白质和病毒消化液中被高灵敏度地检测到。(结论 2023 年末，JN.1（BA.2.86.1.1）免疫逃避亚变异株在全球的流行率上升，这要求加快并实施在人类样本中检测此类毒株的新策略和方案。

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Rapid identification of SARS CoV-2 omicron sub-variant JN.1 (BA.2.86.1.1) with mass spectrometry

Objective

The rapid detection and differentiation of strains of the BA.2.86 lineage including the new sub-variant JN.1 (BA.2.86.1.1) is demonstrated employing selected ion monitoring (SIM) and high resolution mass spectrometry.

Methods

A study of a preliminary set of BA.2.86 lineage positive specimens, identified BA.2.86 and BA.2.86.1.1 peptide markers in 62.5 % and 29.1 % of samples.

Results

Peptide-specific markers in the surface spike protein associated with the L455S mutation are confidently detected with high sensitivity in protein and virus digests.

The virus was thus confidently assigned in over 91 % of positive specimens.

Conclusions

A rise in the global prevalence of the JN.1 (BA.2.86.1.1) immune evasive sub-variant, that emerged in late 2023, requires that new strategies and protocols to detect such strains in human specimens are accelerated and implemented.

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