一种保守转录因子的磷酸代码是真菌毒力的基础。

IF 4.4 1区 生物学 Q1 BIOLOGY BMC Biology Pub Date : 2024-08-26 DOI:10.1186/s12915-024-01978-y
Jiyun Yang, Bing Li, Yu-Ting Pan, Ping Wang, Mei-Ling Sun, Ki-Tae Kim, Hui Sun, Jian-Ren Ye, Zhen Jiao, Yong-Hwan Lee, Lin Huang
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引用次数: 0

摘要

背景:细胞壁完整性(CWI)对真菌生长、致病和适应细胞外环境至关重要。氟化钙(CFW)是一种抑制真菌生长的细胞壁扰动剂,但人们对植物病原真菌如何应对氟化钙诱导的压力知之甚少:结果:在这项研究中,我们发现了一个重大发现,即 CFW 触发了球孢子菌转录因子 CgCrzA 从细胞质到细胞核的转位。这种转位受一个相互作用蛋白 CgMkk1 的调控,CgMkk1 是一种参与 CWI 途径的丝裂原活化蛋白激酶。进一步分析表明,CgMkk1 通过使 CgCrzA 的 Ser280 残基磷酸化来促进核转位。通过染色质免疫沉淀测序,我们确定了 CgCrzA 的两个下游靶标,即 CgCHS5 和 CgCHS6,它们作为几丁质合成酶基因对生长、细胞壁完整性和致病性至关重要:这些发现为 CgMkk1-CgCrzA-CgChs5/6 的调控机制提供了一个新的视角,该调控机制能使细胞壁抑制剂 CFW 起作用并促进球孢子菌的感染性生长。
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Phospho-code of a conserved transcriptional factor underpins fungal virulence.

Background: Cell wall integrity (CWI) is crucial for fungal growth, pathogenesis, and adaptation to extracellular environments. Calcofluor white (CFW) is a cell wall perturbant that inhibits fungal growth, yet little is known about how phytopathogenic fungi respond to the CFW-induced stress.

Results: In this study, we unveiled a significant discovery that CFW triggered the translocation of the transcription factor CgCrzA from the cytoplasm to the nucleus in Colletotrichum gloeosporioides. This translocation was regulated by an interacting protein, CgMkk1, a mitogen-activated protein kinase involved in the CWI pathway. Further analysis revealed that CgMkk1 facilitated nuclear translocation by phosphorylating CgCrzA at the Ser280 residue. Using chromatin immunoprecipitation sequencing, we identified two downstream targets of CgCrzA, namely CgCHS5 and CgCHS6, which are critical for growth, cell wall integrity, and pathogenicity as chitin synthase genes.

Conclusions: These findings provide a novel insight into the regulatory mechanism of CgMkk1-CgCrzA-CgChs5/6, which enables response of the cell wall inhibitor CFW and facilitates infectious growth for C. gloeosporioides.

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来源期刊
BMC Biology
BMC Biology 生物-生物学
CiteScore
7.80
自引率
1.90%
发文量
260
审稿时长
3 months
期刊介绍: BMC Biology is a broad scope journal covering all areas of biology. Our content includes research articles, new methods and tools. BMC Biology also publishes reviews, Q&A, and commentaries.
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