就正畸适应症而言,压缩力对不同间充质干细胞类型的影响。

IF 5.4 2区 医学 Q1 CELL & TISSUE ENGINEERING Stem Cells Translational Medicine Pub Date : 2024-10-10 DOI:10.1093/stcltm/szae057
Chloé Radermacher, Rogerio B Craveiro, Wilhelm Jahnen-Dechent, Justus P Beier, Astrid Bülow, Michael Wolf, Sabine Neuss
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引用次数: 0

摘要

干细胞,例如上颌骨上牙周韧带干细胞(u-PDLSC)和下颌骨上牙周韧带干细胞(l-PDLSC)、脂肪间充质干细胞(AD-MSC)和骨髓间充质干细胞(BM-MSC),在牙周重塑和正畸治疗方面的潜力具有重要意义。在这项工作中,我们重点研究了不同类型干细胞对机械力的综合适应性,目的是更好地理解细胞行为,并完善研究牙周重塑的新机制方法。我们对干细胞进行了全面分析,观察到不同干细胞类型在受压情况下的不同形态和增殖变化。细胞外信号调节激酶(ERK)和蛋白激酶B(又称AKT)的细胞信号传导及其各自的磷酸化对挤压表现出不同的反应。此外,在机械压力下,所有细胞类型的血管内皮生长因子和肝细胞生长因子分泌都会减少,但也有细胞特异性差异。骨蛋白激酶的分泌在挤压下减少,尤其是在u-PDLSC中。至少,在压力下观察到不同类型细胞的NF-kB配体可溶性受体分泌模式各不相同,为了解骨代谢提供了重要信息。这些发现加深了人们对间充质干细胞在机械刺激下行为的理解,突出了它们在正畸和再生医学背景下骨重塑、伤口愈合和组织再生中的作用。我们的研究结果强调了u-PDLSC、l-PDLSC和AD-MSC在牙周再生中的潜力,其中AD-MSC在压缩条件下表现出显著的恢复力,这表明它在正畸研究中的作用值得进一步研究。虽然这些发现令人鼓舞,但要充分理解基于干细胞的牙周疗法的机制,进一步的研究是必不可少的。
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Impact of compression forces on different mesenchymal stem cell types regarding orthodontic indication.

The potential of stem cells, for example upper periodontal ligament stem cells from the maxilla (u-PDLSC) and from the mandible (l-PDLSC), adipose-derived mesenchymal stem cells (AD-MSC), and bone marrow-derived mesenchymal stem cells (BM-MSC), with respect to periodontal remodeling and orthodontic treatment is of great importance. In this work, we focus on the comprehensive adaptability of different stem cell types to mechanical forces with the aim to better understanding cell behavior and to refine a new mechanistic approach to investigate periodontal remodeling. We comprehensively analyze stem cells and observe distinct morphological and proliferation changes under compression in dependence on stem cell type. The cell signaling of extracellular signal-regulated kinase (ERK) and protein kinase B, also called AKT, and their respective phosphorylation shows diverse responses to compression. Additionally, vascular endothelial growth factor and hepatocyte growth factor secretion were reduced under mechanical stress in all cell types, with cell-specific variations. Osteoprotegerin secretion was reduced under compression, particularly in u-PDLSC. At least, diverse soluble receptors of NF-kB-ligand secretion patterns among cell types under pressure were observed, providing crucial insights into bone metabolism. These findings offer a deeper understanding of the behavior of mesenchymal stem cells under mechanical stimuli, highlighting their roles in bone remodeling, wound healing, and tissue regeneration in orthodontic and regenerative medicine contexts. Our results underscore the potential of u-PDLSC, l-PDLSC, and AD-MSC in periodontal regeneration, with AD-MSC showing notable resilience under compression, indicating its promising role for further investigation for orthodontic research. While these findings are encouraging, further research is essential to fully comprehend the mechanism of stem cell-based periodontal therapies.

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来源期刊
Stem Cells Translational Medicine
Stem Cells Translational Medicine CELL & TISSUE ENGINEERING-
CiteScore
12.90
自引率
3.30%
发文量
140
审稿时长
6-12 weeks
期刊介绍: STEM CELLS Translational Medicine is a monthly, peer-reviewed, largely online, open access journal. STEM CELLS Translational Medicine works to advance the utilization of cells for clinical therapy. By bridging stem cell molecular and biological research and helping speed translations of emerging lab discoveries into clinical trials, STEM CELLS Translational Medicine will help move applications of these critical investigations closer to accepted best patient practices and ultimately improve outcomes. The journal encourages original research articles and concise reviews describing laboratory investigations of stem cells, including their characterization and manipulation, and the translation of their clinical aspects of from the bench to patient care. STEM CELLS Translational Medicine covers all aspects of translational cell studies, including bench research, first-in-human case studies, and relevant clinical trials.
期刊最新文献
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