胺氧化酶、含铜 3 (Aoc3) 基因敲除小鼠更易患 DSS 诱导的结肠炎和结肠肿瘤发生。

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL In vivo Pub Date : 2024-09-01 DOI:10.21873/invivo.13695

Özge Özcan, Özge Akyol, Aytekin Akyol

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引用次数: 0

摘要

背景/目的：炎症性肠病和结直肠癌是发病和死亡的主要原因。含铜胺氧化酶 3（AOC3）是白细胞生理迁移和炎症调控的关键酶。本研究旨在探讨 Aoc3 缺乏对小鼠结肠炎和结直肠肿瘤发生模型的影响：用 C57BL/6 小鼠和 Aoc3 基因敲除小鼠进行右旋糖酐硫酸钠（DSS）诱导的急性结肠炎和偶氮甲烷（AOM）/DSS 模型的炎症相关结肠癌研究。我们还评估了 Aoc3 在 Apc 突变小鼠肠道和结肠肿瘤发生模型中的作用：结果：我们观察到，Aoc3缺陷小鼠在DSS诱发结肠炎的早期阶段更易患结肠炎，且存活时间更短。我们还发现，在AOM/DSS和Apc突变小鼠模型中，Aoc3缺陷小鼠发生的肿瘤更多。此外，Aoc3突变小鼠AOM/DSS组的结肠肿瘤一般为浸润型腺癌：结论：Aoc3 缺乏会促进小鼠模型中结肠炎和结肠肿瘤的发生。

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Amine Oxidase, Copper Containing 3 (Aoc3) Knockout Mice Are More Prone to DSS-induced Colitis and Colonic Tumorigenesis.

Background/aim: Inflammatory bowel diseases and colorectal cancer are a major cause of morbidity and mortality. Amine oxidase, copper-containing 3 (AOC3) is a critical enzyme in the physiological trafficking of leukocytes and the regulation of inflammation. This study aimed to examine the effects of Aoc3 deficiency in mice models of colitis and colorectal tumorigenesis.

Materials and methods: C57BL/6 and Aoc3 knockout mice were used for Dextran Sodium Sulfate (DSS) induced acute colitis and the Azoxymethane (AOM)/DSS model of inflammation-related colon cancer. We also evaluated the effect of Aoc3 in an Apc mutant mice model of intestinal and colonic tumorigenesis.

Results: We observed that Aoc3 deficient mice were more prone to colitis induced by DSS in early phases and their survival was shorter. We also showed that Aoc3 deficient mice developed more tumors both in AOM/DSS and Apc mutant mice models. Furthermore, colonic tumors in the AOM/DSS groups in Aoc3 mutant mice were generally invasive type adenocarcinomas.

Conclusion: Aoc3 deficiency promotes colitis and colonic tumorigenesis in mouse models.

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.