不受光学散射影响的低频相干拉曼成像技术

David R. Smith, Jesse W. Wilson, Siddarth Shivkumar, Hervé Rigneault and Randy A. Bartels*, 
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引用次数: 0

摘要

我们展示了低频干涉脉冲刺激拉曼散射(ISRS)成像技术,该技术对光学散射造成的失真具有很高的鲁棒性。ISRS 是一种泵浦-探针相干拉曼光谱,可以捕捉拉曼振动光谱。记录 ISRS 光谱需要将探针脉冲从泵脉冲中分离出来。虽然这种分离在液体等非散射样本中很简单,但散射会导致泵脉冲严重污染,并阻碍拉曼光谱的提取。我们介绍了一种适用于复杂散射样品的 ISRS 显微镜稳健方法。即使泵脉冲和探针脉冲穿过许多散射层,也能获得高信噪比的 ISRS 光谱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Low-Frequency Coherent Raman Imaging Robust to Optical Scattering

We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump–probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.

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来源期刊
Chemical & Biomedical Imaging
Chemical & Biomedical Imaging 化学与生物成像-
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期刊介绍: Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging
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