David R. Smith, Jesse W. Wilson, Siddarth Shivkumar, Hervé Rigneault and Randy A. Bartels*,
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Low-Frequency Coherent Raman Imaging Robust to Optical Scattering
We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump–probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.
期刊介绍：
Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging
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