Qingyi Liu, Qiongdan Zhang, Yuting Zhang, Fanghong Tian, Kang Long, Yupei Yang, Wei Wang, Caiyun Peng and Huizhen Wang
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引用次数: 0
摘要
与单足 DNA 步行器相比，双足 DNA 步行器具有更宽的步行区域和更快的步行动力学，从而提高了扩增效率。在这项研究中，我们设计了一种随机三维（3D）双足 DNA 步行器，它能够在基于 AuNP 的三维轨道上航行，并由外切酶 III（Exo III）驱动。该检测系统能线性检测非侵入性生物标志物嘌呤/嘧啶内切酶 1（APE1）的活性，检测范围为每毫升 0 至 120 U，检测限为每毫升 0.03 U。该平台不仅为细胞裂解物中 APE1 的灵敏检测提供了一种新型 DNA 步行器，还有助于精确评估 NCA 对 APE1 的抑制作用。这项研究为今后筛选其他潜在的 APE1 抑制剂带来了希望。
A recognition-induced three-dimensional bipedal DNA walker for highly sensitive detection of APE1†
In contrast to the unipedal DNA walker, a bipedal DNA walker features a broader walking area and exhibits faster walking kinetics, leading to enhanced amplification efficiency. In this study, we designed a stochastic three-dimensional (3D) bipedal DNA walker, capable of navigating AuNP-based 3D tracks, driven by exonuclease III (Exo III). This detection system enables the linear detection of the non-invasive biomarker apurinic/apyrimidinic endonuclease 1 (APE1) activity across a range of 0 to 120 U per mL, with a detection limit of 0.03 U per mL. The platform not only offers a novel DNA walker for sensitive APE1 detection in cell lysate but also facilitates the precise assessment of NCA's inhibitory effect on APE1. This research holds promise for future screening of other potential APE1 inhibitors.
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