Panicum maximum Jacq.介导的银纳米粒子的绿色合成:分子对接支持的合成、表征和生物活性。

IF 4.5 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2024-12-01 Epub Date: 2024-08-28 DOI:10.1080/21691401.2024.2395811
Heba W Alhamdi, Hanan Khalaf Anazi, Fatma Alzahraa Mokhtar, Seham S Elhawary, Serag Eldin I Elbehairi, Mohammad Y Alfaifi, Ali A Shati, Lamiaa I Fahmy, Engy Elekhnawy, Afnan Hassan, Walaa A Negm, Sherif Ashraf Fahmy, Nabil Selim
{"title":"Panicum maximum Jacq.介导的银纳米粒子的绿色合成:分子对接支持的合成、表征和生物活性。","authors":"Heba W Alhamdi, Hanan Khalaf Anazi, Fatma Alzahraa Mokhtar, Seham S Elhawary, Serag Eldin I Elbehairi, Mohammad Y Alfaifi, Ali A Shati, Lamiaa I Fahmy, Engy Elekhnawy, Afnan Hassan, Walaa A Negm, Sherif Ashraf Fahmy, Nabil Selim","doi":"10.1080/21691401.2024.2395811","DOIUrl":null,"url":null,"abstract":"<p><p>This study uses the aerial parts of <i>Panicum maximum</i> total extract (PMTE) to synthesize silver nanoparticles (AgNPs) in an environmentally friendly manner. TEM, SEM, FTIR, X-ray powder diffraction (XRD), Zeta potential, UV, and FTIR were used to characterize the green silver nanoparticles (PM-AgNPs). PM-AgNPs were evaluated as anticancer agents compared to (PMTE) against breast (MCF-7), lung (A549), and ovary adenocarcinoma (SKOV3) human tumour cells. The antibacterial activity of AgNPs was assessed against <i>Staphylococcus aureus</i> isolates. The PM-AgNPs had an absorbance of 418 nm, particle size of 15.18 nm, and zeta potential of -22.4 mV, ensuring the nanosilver's stability. XRD evaluated the crystallography nature of the formed PM-AgNPs. The cytotoxic properties of PM-AgNPs on MCF-7 and SKOV 3 were the strongest, with IC50s of 0.13 ± 0.015 and 3.5 ± 0.5 g/ml, respectively, as compared to A549 (13 ± 3.2 µg/mL). The increase in the apoptotic cells was 97.79 ± 1.61 and 96.6 ± 1.91% for MCF-7 and SKOV3 cell lines, respectively. PM-AgNPs were found to affect the membrane integrity and membrane permeability of 50 and 43.75% of the tested isolates, respectively. Also, PM-AgNPs have recorded a reduction in the biofilm formation of <i>S. aurues</i>. These results suggest using PM-AgNPs to treat breast and ovarian cancers.</p>","PeriodicalId":8736,"journal":{"name":"Artificial Cells, Nanomedicine, and Biotechnology","volume":"52 1","pages":"411-425"},"PeriodicalIF":4.5000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Panicum maximum</i> Jacq. mediated green synthesis of silver nanoparticles: synthesis, characterization, and biological activities supported by molecular docking.\",\"authors\":\"Heba W Alhamdi, Hanan Khalaf Anazi, Fatma Alzahraa Mokhtar, Seham S Elhawary, Serag Eldin I Elbehairi, Mohammad Y Alfaifi, Ali A Shati, Lamiaa I Fahmy, Engy Elekhnawy, Afnan Hassan, Walaa A Negm, Sherif Ashraf Fahmy, Nabil Selim\",\"doi\":\"10.1080/21691401.2024.2395811\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study uses the aerial parts of <i>Panicum maximum</i> total extract (PMTE) to synthesize silver nanoparticles (AgNPs) in an environmentally friendly manner. TEM, SEM, FTIR, X-ray powder diffraction (XRD), Zeta potential, UV, and FTIR were used to characterize the green silver nanoparticles (PM-AgNPs). PM-AgNPs were evaluated as anticancer agents compared to (PMTE) against breast (MCF-7), lung (A549), and ovary adenocarcinoma (SKOV3) human tumour cells. The antibacterial activity of AgNPs was assessed against <i>Staphylococcus aureus</i> isolates. The PM-AgNPs had an absorbance of 418 nm, particle size of 15.18 nm, and zeta potential of -22.4 mV, ensuring the nanosilver's stability. XRD evaluated the crystallography nature of the formed PM-AgNPs. The cytotoxic properties of PM-AgNPs on MCF-7 and SKOV 3 were the strongest, with IC50s of 0.13 ± 0.015 and 3.5 ± 0.5 g/ml, respectively, as compared to A549 (13 ± 3.2 µg/mL). The increase in the apoptotic cells was 97.79 ± 1.61 and 96.6 ± 1.91% for MCF-7 and SKOV3 cell lines, respectively. PM-AgNPs were found to affect the membrane integrity and membrane permeability of 50 and 43.75% of the tested isolates, respectively. Also, PM-AgNPs have recorded a reduction in the biofilm formation of <i>S. aurues</i>. These results suggest using PM-AgNPs to treat breast and ovarian cancers.</p>\",\"PeriodicalId\":8736,\"journal\":{\"name\":\"Artificial Cells, Nanomedicine, and Biotechnology\",\"volume\":\"52 1\",\"pages\":\"411-425\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Artificial Cells, Nanomedicine, and Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1080/21691401.2024.2395811\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/28 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial Cells, Nanomedicine, and Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/21691401.2024.2395811","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/28 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

本研究利用最大连翘的气生部分总提取物(PMTE)以环保的方式合成银纳米粒子(AgNPs)。利用 TEM、SEM、FTIR、X 射线粉末衍射 (XRD)、Zeta 电位、紫外线和 FTIR 对绿色银纳米粒子(PM-AgNPs)进行了表征。评估了 PM-AgNPs 与 PMTE 相比对乳腺癌(MCF-7)、肺癌(A549)和卵巢腺癌(SKOV3)人类肿瘤细胞的抗癌作用。评估了 AgNPs 对金黄色葡萄球菌分离物的抗菌活性。PM-AgNPs 的吸光度为 418 nm,粒径为 15.18 nm,Zeta 电位为 -22.4 mV,确保了纳米银的稳定性。XRD 评估了所形成的 PM-AgNPs 的晶体学性质。PM-AgNPs 对 MCF-7 和 SKOV 3 的细胞毒性最强,IC50 值分别为 0.13 ± 0.015 和 3.5 ± 0.5 g/ml,而对 A549 的 IC50 值为 13 ± 3.2 µg/mL。MCF-7 和 SKOV3 细胞系的凋亡细胞增加率分别为 97.79 ± 1.61% 和 96.6 ± 1.91%。研究发现,PM-AgNPs 分别影响了 50% 和 43.75% 受测分离物的膜完整性和膜渗透性。此外,PM-AgNPs 还能减少 S. aurues 的生物膜形成。这些结果建议使用 PM-AgNPs 治疗乳腺癌和卵巢癌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Panicum maximum Jacq. mediated green synthesis of silver nanoparticles: synthesis, characterization, and biological activities supported by molecular docking.

This study uses the aerial parts of Panicum maximum total extract (PMTE) to synthesize silver nanoparticles (AgNPs) in an environmentally friendly manner. TEM, SEM, FTIR, X-ray powder diffraction (XRD), Zeta potential, UV, and FTIR were used to characterize the green silver nanoparticles (PM-AgNPs). PM-AgNPs were evaluated as anticancer agents compared to (PMTE) against breast (MCF-7), lung (A549), and ovary adenocarcinoma (SKOV3) human tumour cells. The antibacterial activity of AgNPs was assessed against Staphylococcus aureus isolates. The PM-AgNPs had an absorbance of 418 nm, particle size of 15.18 nm, and zeta potential of -22.4 mV, ensuring the nanosilver's stability. XRD evaluated the crystallography nature of the formed PM-AgNPs. The cytotoxic properties of PM-AgNPs on MCF-7 and SKOV 3 were the strongest, with IC50s of 0.13 ± 0.015 and 3.5 ± 0.5 g/ml, respectively, as compared to A549 (13 ± 3.2 µg/mL). The increase in the apoptotic cells was 97.79 ± 1.61 and 96.6 ± 1.91% for MCF-7 and SKOV3 cell lines, respectively. PM-AgNPs were found to affect the membrane integrity and membrane permeability of 50 and 43.75% of the tested isolates, respectively. Also, PM-AgNPs have recorded a reduction in the biofilm formation of S. aurues. These results suggest using PM-AgNPs to treat breast and ovarian cancers.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Artificial Cells, Nanomedicine, and Biotechnology
Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
10.90
自引率
0.00%
发文量
48
审稿时长
20 weeks
期刊介绍: Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.
期刊最新文献
Zingiberis rhizoma-based carbon dots alter serum oestradiol and follicle-stimulating hormone levels in female mice. HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones. Inhibitory effects of Curcumae Radix carbonisata-based carbon dots against liver fibrosis induced by carbon tetrachloride in mice. Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds - preparation, characterisation, and evaluation. Investigation of pharmacokinetics and immunogenicity of magnetosomes.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1