基于分子技术确认阿根廷暴发的旋毛虫病与美洲狮肉香肠有关

IF 5 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY International journal of food microbiology Pub Date : 2024-08-24 DOI:10.1016/j.ijfoodmicro.2024.110889
Silvio J. Krivokapich , M. Fernanda Degese , Graciana M. Gatti , Laura Lopez , María A. Simonetti , María C. Crescia , Claudio D. Calvo , Cecilia L. Lapuyade , Natalia Cazaux , Florencia A. D'Francisco
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引用次数: 0

摘要

旋毛虫病是一种由旋毛虫引起的人畜共患疾病,主要传染源是食用猪肉和猪肉衍生产品。不过,也可通过食用体育狩猎对象野生动物的肉而感染。本研究的目标是1)开发并实施一种分子方法,用于鉴定生吃的香肠中的猪(Sus scrofa)和美洲狮(Puma concolor)肉,这些香肠分别于 2010 年、2021 年和 2022 年在阿根廷科尔多瓦省、布宜诺斯艾利斯省和拉潘帕省暴发旋毛虫病;以及 2)鉴定食物中的旋毛虫种类。我们设计了特定的引物,用于对两种宿主的线粒体细胞色素 b 基因的一个区域进行 PCR 扩增和核苷酸测序。对上述疫情的样本进行了分析,并对毛旋毛虫幼虫进行了分子鉴定。物种鉴定系统的结果显示,科尔多瓦和布宜诺斯艾利斯的香肠混合了猪肉和美洲狮肉,而拉潘帕的香肠只含有美洲狮肉。这三起疫情都与螺旋毛癣菌(Trichinella spiralis)有关。这项研究开发和实施的物种鉴定系统发现了两种与人类病例有关的旋毛虫宿主物种,并提醒人们注意通过受感染美洲狮肉向人类传播人畜共患病的风险。
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Molecular based confirmation of puma meat sausages implicated in trichinellosis outbreaks in Argentina

Trichinellosis is a zoonotic disease caused by Trichinella, with the main source of infection being the consumption of pork and pork-derived products. However, it can also be acquired from eating the meat from wild animals targeted for sport hunting. The objectives of this study were: 1) to develop and implement a molecular method for the identification of Sus scrofa (pig and wild boar) and Puma concolor (Puma) meat in sausages eaten raw, which were linked to trichinellosis outbreaks occurring in Córdoba, Buenos Aires and La Pampa provinces, Argentina, in 2010, 2021, and 2022, respectively; and 2) to identify the Trichinella species present in the food. Specific primers were designed for PCR amplification and nucleotide sequencing of a region of the mitochondrial cytochrome b gene from both host species. Samples from the mentioned outbreaks were analysed, and the molecular identification of Trichinella spp. larvae was also performed. Results of the species identification system revealed that sausages from Córdoba and Buenos Aires had a mixed composition of pork and puma meat, while those from La Pampa contained puma meat only. Trichinella spiralis was implicated in all three outbreaks. The species identification system developed and implemented in this study revealed two host species of Trichinella related to human cases, and alerts about the risk of zoonotic transmission to humans through infected puma meat.

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来源期刊
International journal of food microbiology
International journal of food microbiology 工程技术-食品科技
CiteScore
10.40
自引率
5.60%
发文量
322
审稿时长
65 days
期刊介绍: The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.
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