神经元的激活会影响核斑点的组织和蛋白质组成。

IF 4.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-08-26 DOI:10.1016/j.bbamcr.2024.119829
Andrzej Antoni Szczepankiewicz , Kamil Parobczak , Monika Zaręba-Kozioł , Błażej Ruszczycki , Monika Bijata , Paweł Trzaskoma , Grzegorz Hajnowski , Dagmara Holm-Kaczmarek , Jakub Włodarczyk , Hanna Sas-Nowosielska , Grzegorz Marek Wilczyński , Maria Jolanta Rędowicz , Adriana Magalska
{"title":"神经元的激活会影响核斑点的组织和蛋白质组成。","authors":"Andrzej Antoni Szczepankiewicz ,&nbsp;Kamil Parobczak ,&nbsp;Monika Zaręba-Kozioł ,&nbsp;Błażej Ruszczycki ,&nbsp;Monika Bijata ,&nbsp;Paweł Trzaskoma ,&nbsp;Grzegorz Hajnowski ,&nbsp;Dagmara Holm-Kaczmarek ,&nbsp;Jakub Włodarczyk ,&nbsp;Hanna Sas-Nowosielska ,&nbsp;Grzegorz Marek Wilczyński ,&nbsp;Maria Jolanta Rędowicz ,&nbsp;Adriana Magalska","doi":"10.1016/j.bbamcr.2024.119829","DOIUrl":null,"url":null,"abstract":"<div><p>Nuclear speckles, also known as interchromatin granule clusters (IGCs), are subnuclear domains highly enriched in proteins involved in transcription and mRNA metabolism and, until recently, have been regarded primarily as their storage and modification hubs. However, several recent studies on non-neuronal cell types indicate that nuclear speckles may directly contribute to gene expression as some of the active genes have been shown to associate with these structures.</p><p>Neuronal activity is one of the key transcriptional regulators and may lead to the rearrangement of some nuclear bodies. Notably, the impact of neuronal activation on IGC/nuclear speckles organization and function remains unexplored. To address this research gap, we examined whether and how neuronal stimulation affects the organization of these bodies in granular neurons from the rat hippocampal formation. Our findings demonstrate that neuronal stimulation induces morphological and proteomic remodelling of the nuclear speckles under both <em>in vitro</em> and <em>in vivo</em> conditions. Importantly, these changes are not associated with cellular stress or cell death but are dependent on transcription and splicing.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 8","pages":"Article 119829"},"PeriodicalIF":4.6000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0167488924001721/pdfft?md5=531bf475ffb59aed7ac2dcaedf185d6f&pid=1-s2.0-S0167488924001721-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Neuronal activation affects the organization and protein composition of the nuclear speckles\",\"authors\":\"Andrzej Antoni Szczepankiewicz ,&nbsp;Kamil Parobczak ,&nbsp;Monika Zaręba-Kozioł ,&nbsp;Błażej Ruszczycki ,&nbsp;Monika Bijata ,&nbsp;Paweł Trzaskoma ,&nbsp;Grzegorz Hajnowski ,&nbsp;Dagmara Holm-Kaczmarek ,&nbsp;Jakub Włodarczyk ,&nbsp;Hanna Sas-Nowosielska ,&nbsp;Grzegorz Marek Wilczyński ,&nbsp;Maria Jolanta Rędowicz ,&nbsp;Adriana Magalska\",\"doi\":\"10.1016/j.bbamcr.2024.119829\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Nuclear speckles, also known as interchromatin granule clusters (IGCs), are subnuclear domains highly enriched in proteins involved in transcription and mRNA metabolism and, until recently, have been regarded primarily as their storage and modification hubs. However, several recent studies on non-neuronal cell types indicate that nuclear speckles may directly contribute to gene expression as some of the active genes have been shown to associate with these structures.</p><p>Neuronal activity is one of the key transcriptional regulators and may lead to the rearrangement of some nuclear bodies. Notably, the impact of neuronal activation on IGC/nuclear speckles organization and function remains unexplored. To address this research gap, we examined whether and how neuronal stimulation affects the organization of these bodies in granular neurons from the rat hippocampal formation. Our findings demonstrate that neuronal stimulation induces morphological and proteomic remodelling of the nuclear speckles under both <em>in vitro</em> and <em>in vivo</em> conditions. Importantly, these changes are not associated with cellular stress or cell death but are dependent on transcription and splicing.</p></div>\",\"PeriodicalId\":8754,\"journal\":{\"name\":\"Biochimica et biophysica acta. Molecular cell research\",\"volume\":\"1871 8\",\"pages\":\"Article 119829\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0167488924001721/pdfft?md5=531bf475ffb59aed7ac2dcaedf185d6f&pid=1-s2.0-S0167488924001721-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et biophysica acta. Molecular cell research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167488924001721\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et biophysica acta. Molecular cell research","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167488924001721","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

核斑点又称染色质间颗粒簇(IGCs),是参与转录和 mRNA 代谢的蛋白质高度富集的核下结构域,直到最近,核斑点一直被认为主要是它们的储存和修饰中心。然而,最近几项关于非神经元细胞类型的研究表明,核斑点可能直接促进基因表达,因为一些活性基因已被证明与这些结构有关联。神经元活动是关键的转录调节因子之一,可能导致一些核体的重新排列。值得注意的是,神经元活化对 IGC/核斑点组织和功能的影响仍有待探索。为了填补这一研究空白,我们研究了神经元刺激是否以及如何影响大鼠海马形成颗粒神经元中这些核体的组织。我们的研究结果表明,在体外和体内条件下,神经元刺激会诱导核斑点的形态学和蛋白质组重塑。重要的是,这些变化与细胞压力或细胞死亡无关,而是依赖于转录和剪接。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Neuronal activation affects the organization and protein composition of the nuclear speckles

Nuclear speckles, also known as interchromatin granule clusters (IGCs), are subnuclear domains highly enriched in proteins involved in transcription and mRNA metabolism and, until recently, have been regarded primarily as their storage and modification hubs. However, several recent studies on non-neuronal cell types indicate that nuclear speckles may directly contribute to gene expression as some of the active genes have been shown to associate with these structures.

Neuronal activity is one of the key transcriptional regulators and may lead to the rearrangement of some nuclear bodies. Notably, the impact of neuronal activation on IGC/nuclear speckles organization and function remains unexplored. To address this research gap, we examined whether and how neuronal stimulation affects the organization of these bodies in granular neurons from the rat hippocampal formation. Our findings demonstrate that neuronal stimulation induces morphological and proteomic remodelling of the nuclear speckles under both in vitro and in vivo conditions. Importantly, these changes are not associated with cellular stress or cell death but are dependent on transcription and splicing.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
10.00
自引率
2.00%
发文量
151
审稿时长
44 days
期刊介绍: BBA Molecular Cell Research focuses on understanding the mechanisms of cellular processes at the molecular level. These include aspects of cellular signaling, signal transduction, cell cycle, apoptosis, intracellular trafficking, secretory and endocytic pathways, biogenesis of cell organelles, cytoskeletal structures, cellular interactions, cell/tissue differentiation and cellular enzymology. Also included are studies at the interface between Cell Biology and Biophysics which apply for example novel imaging methods for characterizing cellular processes.
期刊最新文献
Iron‑sulfur cluster biogenesis and function in Apicomplexa parasites. Targeting SphK1/S1PR3 axis ameliorates sepsis-induced multiple organ injury via orchestration of macrophage polarization and glycolysis. Impaired insulin signaling and diet-induced type 3 diabetes pathophysiology increase amyloid β expression in the Drosophila model of Alzheimer's disease Interplay of force and local mechanisms in axonal plasticity and beyond Oncogenic KRAS mutations modulate BAX-mediated cell death
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1