Soumya Sethi, Tao Xu, Aritra Sarkar, Christoph Drees, Claire Jacob, Andreas Walther
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引用次数: 0
摘要
基于 DNA 的张力探针具有精确可编程的力响应,为细胞机械传感提供了重要的见解。然而,这些探针在细胞培养中的可降解性限制了它们在长期成像中的应用,例如,当细胞迁移、分裂和分化时。这对于深入了解这些长期过程的机械生物学来说是一个关键的限制。在这里,我们展示了完全基于生物 D-DNA 的立体异构体(即 L-DNA)设计的基于 DNA 的张力探针。我们证明,L-DNA 张力探针基本上不受核酸酶的破坏,可进行长达数天的成像,且图像质量不会有明显下降。我们还展示了这些弹性探针在对微小运动成像以及生成单细胞和集体迁移细胞群的长期力图方面的潜力。
Nuclease-Resistant L-DNA Tension Probes Enable Long-Term Force Mapping of Single Cells and Cell Consortia.
DNA-based tension probes with precisely programmableforce response provide important insights into cellularmechanosensing. However, their degradability in cell culture limitstheir use for long-term imaging, for instance, when cells migrate,divide, and differentiate. This is a critical limitation for providinginsights into mechanobiology for these longer-term processes. Here,we present DNA-based tension probes that are entirely designedbased on the stereoisomer of biological D-DNA, i.e., L-DNA. Wedemonstrate that L-DNA tension probes are essentially indestructibleby nucleases and provide days-long imaging without significant lossin image quality. We also show their superiority already for shortimaging times commonly used for classical D-DNA tension probes.We showcase the potential of these resilient probes to image minutemovements, and for generating long term force maps of single cellsand of collectively migrating cell populations.
期刊介绍:
Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.